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Session 2A Imaging and detection: from agents to patients

Chairs: Ivo Vellekoop (BMPI) & Madhumohan Katika (MCBP)


Highly stable zwitterionic nanogels - Tony Ekkelenkamp (BST)

Polymer nanoparticles are promising vehicles for the targeted delivery of drugs or contrast agents. However, protein adsorption limits their circulation time and targeting abilities. Zwitterionic polymers exhibit extremely low protein adsorption. Therefore, a nanogel composed of a zwitterionic polymer was prepared, which showed high stability in protein solution and minimal cell toxicity.
Keywords: polymer, nanogel, colloidal stability


Plasmonic-based sensing of bacterial pathogens - Kristian Goeken (NBP)

Recent emergence of drug resistant pathogens has led to a need for point-of-care devices capable of sensitive on-site detection. We report the development of a DNA/RNA sensor based on localized surface plasmon resonance coupling of nanoparticles and show initial results obtained with crude lysates of M. Tuberculosis and S. Aureus.
Keywords: Nanoparticles, Sensing, Plasmonics


Resonant vaporization of perfluorocarbon phase change agents with surface acoustic waves - Guillaume Lajoinie (PoF)

Acoustic droplet vaporization (ADV) presents great biomedical potential. Droplets can extravesate and the resonance capacity of the vaporized microbubbles makes them ideal ultrasound contrast agents. We propose a new resonance mechanism based on high frequency ultrasound, generated through surface acoustic waves, which allow for achieving ADV at low pressures.
Keywords: ultrasound / contrast agent / vaporization


Absorption and photoacoustic measurements using integrating spheres - Yolanda Villanueva (BMPI)

A method using integrating spheres as platform for simultaneous absorption and photoacoustic measurements with biomedical samples such as blood, joint and cyst fluids from human donors is presented. With the derived equations and developed experimental setup, material properties relevant in quantitative photoacoustics such as absorption coefficient and Grüneisen parameter are measured.
Keywords: Absorption coefficient, Photoacoustics, Grüneisen parameter


Weight adjusted protocols in nuclear medicine, isn`t it time? - Joris van Dijk (RaM/Isala)

Despite decreasing image quality in heavier patients, fixed dose protocols are still the mainstay in many guidelines for imaging procedures. We present a method to overcome this problem by deriving and validating patient tailored protocols using either SPECT, PET or CT. Several studies demonstrating the added value in clinical practice will be presented.
Keywords: imaging, patient-specific, dose

Session 2B: Cells, tissues and organs

Chairs: Jai Prakash (BST) & Jasper van Weerd (DBE)


Raman microscopy: cells, methods and technology - Frank Timmermans (MCBP)

We will present chemical specific microscopic analysis with Raman microscopy. This label free technique enables detailed chemical specificity in diverse fields as cell biology, solid-state physics, and catalysis. Topics of interest in MCBP are single cell analysis, and the integration of Raman microscopy with devices as microfluidics and electron microscopes.
Keywords: Raman microscopy, Single cell analysis, Integrated techniques


Computer Aided Diagnosis in Clinical Pathology - Oscar Geessink (RaM)

Treatment selection for cancer patients is largely based on the result of microscopic tissue inspection. However, the visual interpretation by pathologists is subjective, variable and limited. In the pursuit of personalized medicine, there is need for advanced and more reliable tissue diagnostics. Digital Pathology will meet this need. 
Keywords: Image analysis, Machine learning, Histology


Development of bioactive membrane for bioartificial kidney device (BAK) - Natalia Chevtchik (BST)

Protein bound toxins can’t be removed by conventional membranes during hemodialysis therapies. A BAK containing renal cells with functional transporters could achieve this. We investigate strategies for obtaining a “living membrane” composed of tight renal cell monolayer on a functionalized polymeric membrane. This is the first important step towards developing a BAK. 
Keywords: Bioartificial kidney device, Functionalized membranes


Immuno-Protective Membrane-Based Scaffolds For Extra Hepatic Islet Transplantation - Milou Groot Nibbelink (DBE)

In clinical islet of Langerhans transplantation 60-70% of the donor islets are lost immediately after transplantation. This is due to mechanical stress, different immune-responses, and lack of vascularization. The aim of our study is to improve the transplantation outcome by immune-protection of islets, prevascularization, and oxygen generation using macro-encapsulation devices.
Keywords: Diabetes, Transplantation, Immuno-protection


Targeted Therapeutics: Aiming at the resolution of liver fibrosis - Ruchi Bansal (BST)

Liver fibrosis is a growing cause of mortality worldwide. Hepatic stellate cells and macrophages are the key cell-types that contribute to the initiation and progression of fibrosis. Therefore, we are aiming at the development of innovative targeted therapeutics for the selective inhibition of these disease-inducing cell-types for the resolution of liver diseases.
Key words: liver fibrosis, targeted therapeutics, pathogenic cells