HomeEducationDoctorate (PhD & PDEng)Current candidatesPhD infoUpcoming Public DefencesPhD Defence Sandrien Verloy | Extending the microfabrication and particle handling toolbox for the development of ordered chromatography columns

PhD Defence Sandrien Verloy | Extending the microfabrication and particle handling toolbox for the development of ordered chromatography columns

Extending the microfabrication and particle handling toolbox for the development of ordered chromatography columns

The PhD defence of Sandrien Verloy will take place (partly) online and can be followed by a live stream.

Sandrien Verloy is a PhD student in the research group Mesoscale Chemical Systems (MCS). Supervisors are prof.dr. J.G.E. Gardeniers from the Faculty of Science & Technology (S&T) and prof.dr. G. Desmet (Free University of Brussels, Belgium).

Improving the order of the beads packed in a liquid chromatography column would practically translate into a situation where for instance a researcher in the biomedical field analyzing a biological sample (e.g. a cancer cell) would be able to identify a multifold more molecules (eg proteins), using one and the same, yet optimized liquid chromatography column.

The present work investigates a number of new techniques to produce order columns suitable for chromatography. These techniques include a combination of deep reactive ion etching in silicon with mechanical rubbing and other particle handling methods to fill arrays of micro-pockets with either single particles or a well-defined multiple. A broad range of particle handling methods has been explored and has led to a fully optimized procedure involving a novel combination of micro-structured polydimethylsiloxane and wet manual rubbing. During the work, the power of silicon micromachining has also been explored to produce perfectly ordered columns for gas chromatography.