Members of the Nanobiophics group recelty published in ACS Physics of Chemisry. B were the Quantum Efficeinty of Red Fluorescent proteins is measuerd by controlling the single fluorescent optical enviroment. By doing so the real (bright) quantum efficiency appears much higher as expected, The lower respons is is explained by a significant high absorbing non emitting proteins. RFP's appear to have as a significant dark fraction.
This publication is in colloration with researchers of the University of Amsterdam.
The open access publication can be found here: https://doi.org/10.1021/acs.jpcb.9b10396