Liquid chromatography-mass spectrometry (LC-MS) is an analytical chemistry technique that combines the physical separation capabilities of liquid chromatography with the mass analysis capabilities of mass spectrometry. LC-MS is a technique used for many applications which has very high sensitivity and specificity. Generally its application is oriented towards the specific detection and potential identification of chemicals in the presence of other chemicals (in a complex mixture).
Chromatography, Mass spectrometry
Measurements / Setups:
Separation of different components
Ionization and measured mass-to-charge ratio (m/z)
Three interfaces which differ the ionization technique: Electrospray Ionization (ESI), Atmospheric Pressure Chemical Ionization (APCI), and Atmospheric Pressure Photo Ionisatin (APPI). LC have reverse column C18.
H. Hayen, M.M. Alvarez-Grima, S.C. Debnath, J.W.M. Noordermeer,and U. Karst, Analytical Chemistry 76, 1063 (2004)
S. Sarkawi / W.K. Dierkes