Saskia Lindhoud

-Short Biography

After completing a BSc and MSc on Molecular Sciences at Wageningen University in 2005, Saskia Lindhoud started a PhD project at the Laboratory of Physical Chemistry and Colloid Science under supervision of Prof. Martien Cohen Stuart and Prof. Willem Norde. She studied the incorporation of enzymes in polyelectrolyte complex micelles. Within four years (2009) she defended a thesis entitled “Polyelectrolyte Complex Micelles as Wrapping for Enzymes.” After graduating, she left the Netherlands to investigate whether shower-gel can be made “greener” using oxidized cellulose as viscosity modifier. This research was performed at the University of Bath (UK). During this two year project she performed many neutron and x-ray scattering experiments at large scale facilities (ISIS, DIAMOND, ESRF and ILL) trying to resolve the structure of these oxidized cellulose gels.Beginning 2013 she joined the Nanobiophysics group at the University of Twente to work on a project called: “Motor failure in cellular disease.” Later that year she was awarded NWO prestigious Veni award on “Complex Coacervates as Molecular Crowding Agents,” to start her own research line.

-Contact Information -

Dr. Saskia Lindhoud

Nanobiophysics

University of Twente

Institute for Nanotechnology MESA+

Zuidhorst ZH157

Drienerlolaan 5

7522 NB  Enschede, the Netherlands

PO-box 217

7500AE Enschede, the Netherlands

P +31-(0)53-489-3161

F +31-(0)53-489-2150


s.lindhoud @ utwente.nl

-Own Research in NBP-

Complex Coacervates as Molecular Crowding Agents

Complex coacervates are liquid-like structures which form when oppositely charged macromolecules are mixed under the right conditions (ionic strength, pH and mixing ratio). I am interested in the behavior of protein molecules in these dense liquid phases, because I believe that they can serve as a model system for the cytosol or liquid-like components in the cytosol, such as membrane-less organelles. The protein concentration in the complex coacervates can be as high as the protein concentration in the cytosol (200 g/L). We further investigate whether protein molecules remain biological active in these dense phases and study their diffusion and aggregation inside complex coacervates.

Research id: www.researcherid.com/rid/A-5510-2013

Orchid: orcid.org/0000-0002-4164-0763

Google Scholar: https://scholar.google.nl/citations?user=E_mPIv8AAAAJ&hl=en

Researchgate: https://www.researchgate.net/profile/Saskia_Lindhoud

Please check my personal website: https://sites.google.com/site/saskialindhoud/home for news and all my publications

Selected Publications

 

Accumulation of small protein molecules in a macroscopic complex coacervate, S Lindhoud and MMAE Claessens, Soft Matter 2015, DOI:10.1039/C5SM02386F

Relaxation phenomena during polyelectrolyte complex formation, S Lindhoud, MA Cohen Stuart, Advances in Polymer Science, DOI: 10.1007/12_2012_178

Effects of polyelectrolyte complex micelles and their components on the enzymatic activity of lipase, S Lindhoud, W Norde, MA Cohen Stuart, Langmuir 26 (12), 9802-9808

Salt-induced disintegration of lysozyme-containing polyelectrolyte complex micelles, S Lindhoud, L Voorhaar, R de Vries, R Schweins, MA Cohen Stuart, W Norde, Langmuir 25 (19), 11425-11430

Reversibility and relaxation behavior of polyelectrolyte complex micelle formation, S Lindhoud, W Norde, MA Cohen Stuart, The Journal of Physical Chemistry B 113 (16), 5431-5439

Salt-induced release of lipase from polyelectrolyte complex micelles, S Lindhoud, R de Vries, R Schweins, MA Cohen Stuart, W Norde, Soft Matter 5 (1), 242-250

Structure and stability of complex coacervate core micelles with lysozyme, S Lindhoud, R de Vries, W Norde, MA Cohen Stuart, Biomacromolecules 8 (7), 2219-2227

Phase behavior of mixtures of oppositely charged nanoparticles: Heterogeneous Poisson-Boltzmann cell model applied to lysozyme and succinylated lysozyme, PM Biesheuvel, S Lindhoud, R de Vries, MA Cohen Stuart, Langmuir 22 (3), 1291-1300