Dr. R. Gill obtained his B.A. in physics and B.Sc in materials engineering, summa cum laude, from the Technion – Israel institute of Technology. He then did his M. Sc and PhD research in the group of Prof. Itamar Willner at the Hebrew University in Jerusalem. His main research focus was the use of semiconductor nanoparticles (“quantum dots”) for biosensor applications. In 2008 he joined to Philips Research, in Eindhoven as postdoctoral fellow to work on the use of silver nanoparticles for Surface enhanced resonant Raman spectroscopy (SERRS)-based multiplexed detection of pathogens, and in July of 2010 he joined the NBP group at the UT as a postdoctoral fellow. Since 2011 he an assistant professor in the NBP Group.
-Contact Information -
University of Twente
MIRA institute of biomedical technology and technical medicine
7522 NB Enschede, the Netherlands
7500AE Enschede, the Netherlands
TOPIC: Diagnostic applications of metal nanoparticles.
Noble metal nanoparticles exhibit unique optical properties that are different from the properties of the bulk metal. The electric field of light can induce a coherent oscillation of electrons (known as plasmons) on the surface of the particles. Depending on their composition, shape and size, (and also the medium in which the nanoparticles are embedded), a specific resonant frequency exists in which the interaction of light with the localized surface plasmons is maximal. When excited near the plasmon resonance frequency, very strong electromagnetic fields are created near the surface of the nanoparticles. These strong fields can enhance the interaction of light with molecules in the vicinity of the surface, giving rise to phenomena such as surface-enhanced Raman scattering (SERS) and surface-enhanced fluorescence (SEF).
We have been exploring two different concepts of applying plasmon-related phenomena to diagnostic applications. One concept applies the phenomenon of SEF to enhance the detection of biomolecules. Using a 3-part hybridization assay, we have recently shown that we can 200x lower detection limit for a DNA target by using SEF when measured on a conventional fluorescence plate reader. The second concept involves the detection of a DNA target by having the target link a surface bound nanoparticle to a smaller nanoparticle from the solution. This shifts the plasmon resonance peak of the surface bound nanoparticle in a significant way that allows us to detect this change simply by taking a color image with a camera under dark-field illumination. This project includes a wide range of activities, from creation of stable DNA-gold nanoparticle hybrids, through FDTD simulation on the plasmon-plasmon interaction, and up to actual sensing of M. Tuberculosis RNA from bacteria lysates that are provided from our collaborators at the Dutch Royal Tropical Institute (KIT)
A list of publication with citation data can be found at: http://www.researcherid.com/rid/C-7294-2009