The BIOS Lab-on-a-Chip chair (“Miniaturized systems for biomedical and environmental applications”) aims at the research and development of Lab-on-a-Chip (LOC) systems. The BIOS group is embedded within the MIRA institute for biomedical research and technical medicine, and the MESA+ institute for Nanotechnology. MESA+ is one of the world’s largest nanotechnology research institutes; and it’s the largest research institute in this field in the Netherlands. Research in this project will be conducted in close collaboration with the Analytical Biochemistry group (www.biomac.nl) in the field of electrochemistry-mass spectrometry related to the further development of electrochemical, site-specific peptide bond cleavage in proteins, and the screening of new drugs in electrochemical microreactors.
Electrochemistry is a well-studied method for the mimicry of drug metabolism that is complementary to other in vitro techniques like liver microsomal incubations. Additionally, exciting developments have been reported in recent years with respect to protein analysis comprising the electrochemical reduction of disulfide bonds, important for biopharmaceutical protein analysis, and the specific cleavage of peptide bonds, relevant for proteomics.
In this project, the aim is to develop microfluidic chips for in-situ analysis to gain a better understanding of the reactions occurring at the electrode-solution interface. This microfluidic chip will contain ordered nanofabricated structures for surface enhanced Raman spectroscopy (SERS), or structures for surface enhanced infrared absorption spectroscopy (SEIRAS). SEIRAS and SERS are two complementary methods, since vibrations active in SERS are inactive in SEIRAS and vice versa. Moreover, enhancement of the spectroscopic methods is essential to lower the limit of detection.
This PhD position focusses on the device design, fabrication and initial testing. In close collaboration with another PhD and postdoc within this project, proteomics and drug screening applications will be tested in detail. The other PhD position is offered here: http://www.rug.nl/about-us/work-with-us/job-opportunities/overview?cat=phd
For detailed information and to get a better impression of the kind of work in this project, the following papers are recommended:
- Electrochemical protein cleavage in a microreactor: http://eprints.eemcs.utwente.nl/27326/
- Drug screening in a microreactor: http://pubs.rsc.org/-/content/articlehtml/2016/lc/c6lc00708b
In-situ spectroelectrochemistry: http://eprints.eemcs.utwente.nl/26002/
For questions regarding this position contact Mathieu Odijk (firstname.lastname@example.org). Please submit your application using the on-line form at www.utwente.nl/vacatures , including a C.V. and motivation letter. The deadline for submission is February 15th 2017. Please do NOT send your C.V. and motivation letter by email. Postdoctoral research do not need to apply, as we are specifically looking for a prospective PhD student.
We are looking for a candidate with a Master’s Degree in a relevant field, like e.g. electrical engineering, nanotechnology, (analytical) chemistry, chemical engineering, or physics. The candidate should be able to work independently, and have excellent theoretical and experimental skills. Fluent command of English is required. Knowledge or experience in micro- and nanofabrication is considered as a key advantage. Moreover, knowledge in microfluidics, optics/waveguides and spectroscopic methods such as (surface enhanced) Raman and/or Fourier transform infrared spectroscopy is also greatly appreciated.
Most importantly, the BIOS Lab-on-a-Chip group in general, and this project in particular are very challenging multidisciplinary environments. It is therefore essential to show willingness to look beyond disciplinary boundaries, to learn new techniques and skills required for this project.
PhD students are employed for a period of 4 years. Salary and conditions are in accordance with the Collective Labor Agreement (CAO) of the Dutch Universities. The gross monthly salary starts at € 2191,- gross per month in the first year, leading up to € 2801,- gross per month in the last year excluding allowances in accordance with the job profile ‘Promovendus’, under the University System for Job Classification (UJC).
Employees are also entitled to a holiday allowance of 8% of the gross annual salary and a year-end bonus of 8.3 %.
The University of Twente. We stand for life sciences and technology. High tech and human touch. Education and research that matter. New technology which drives change, innovation and progress in society. The University of Twente is the only campus university in the Netherlands; divided over five faculties we provide more than fifty educational programmes. The University of Twente has a strong focus on personal development and talented researchers are given scope for carrying out pioneering research.
The faculty of Electrical Engineering, Mathematics and Computer Science (EEMCS) comprises three disciplines that shape Information and Communication Technology. ICT is more than communication. In almost every product we use mathematics, electronics and computer technology and ICT now contributes to all of societies' activities. The faculty works together intensively with industrial partners and researchers in the Netherlands and abroad and conducts extensive research for external commissioning parties and funders. The research which enjoys a high profile both at home and internationally, has been accommodated in the multidisciplinary research institutes MESA+, CTIT and MIRA.