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Nanobiophysics (NBP)
Nanobiophysics (NBP)
UTFacultiesTNWResearchDept BETNBPLaboratoriesMicroscopy Laboratory

Microscopy laboratory

Nanobiophysics Group uses two main laboratories, the physics laboratory hosts many modern tools to study cell interaction and protein aggregations. Many (custom-build) microscopes are available build for a broad and versatile applications. The microscopy lab ZH174 is restricted area due to the AP-I & LASER  classification 3b, 4. It's allowed to study ML-1 calssified samples in a closed container.

Location [Building 28] Zuidhorst: 1.74

Laboratory contact and safety manager is Robert Molenaar

Keywords: Nanopositioners, lasers, fluorescence, TIRFM total internal reflection microscopy, darkfield, AFM atomic force microscopy, confocal microscopy, fluorescent lifetime, spectroscopy, lightscatter. nanophotonics, LDOS, imaging, 

Overview of microscopy techniques that are available.

Atomic Force microscopy.

  1. Bruker Catalyst AFM for scanning, tapping and peak force imaging, compatible with live cells.  
  2. Multiple top-head design AFM systems, for versatile sample interaction/manipulation by AFM.

FluorescenT Microscopy Techniques

  1. PicoQuant MT200 Laser scanning confcoal microscope with 4 channels: single molecule sensitive applications, confocal imaging, FCS, FCCS, FLIM, fluorescent lifetime, phosphorescence Lifetime, FRET burst analysis, anistropy, antibunching and nanosecond FCS. 
  2. PicoQuant, FCS compatible microscope, dual TCSPC channels.
  3. PicoQuant, FCS compatible microscope, dual TCSPC channels.
  4. Confocal microscope with TCSPC for active Local Density of States modification.
  5. Fianium pulsed light source, full white light spectrum.
  6. Nikon Ti-E TIRF microscope, wide field based for PALM, STORM dual channel widefield/TIRF.
  7. Fluoromax-4 fluorospectrometer with lifetime extension.
  8. Zeiss Axioivert fluorescent microscope.
  9. Nikon TI-2000 fluorescent microscope with live cell incubator.
  10. Nikon TI-2000 Laser excited fluorescent microscope for single particle tracking.
  11. Custom build Dynamic Light Scatter setup DLS (towards Static Light scatter).
  12. Olympus GX71, transmission and reflection compatible darkfield inverted microscope for scattering nano-particles.
  13. Cary Eclipse Spectrophotometer


FastFLIMConfocal imagingLifetime FitsFluorescent Single particle tracking of nanoparticles
Photo: Robert Molenaar
FastFlim Lifetime of membrane potential with FluoVolt dye
FLIM image measured with PicoQuant MT200 LSM. The fluorescent liftime is dependant on cell mebrane potential, excitation 485nm on FluoVolt dye.
Photo: Robert Molenaar
BPAE cells
BPAE cells, imaged with PicoQuant MT200 LSM
3 channel: 405 DAPI, 488 BodyP & 560 MitoTracker Red
Photo: Robert Molenaar
Measurement of the Fluorescent Lifetime
TCSPC Measured exponential decay and and fitted values.
Photo: Robert Molenaar
Fluorescent Single particle tracking of nanoparticles
A dedicated microscope is special set for Fluorescent Single particle tracking of nanoparticles: virus, vesicles, plastics.

Keywords, University, Twente, Netherlands, Nederland, microscopy center, laser

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