Yoonsun Yang

Post Doc

E-mail: y.s.yang@utwente.nl
+31 53 489 5362
+31 53 489 3511
Faculty of Science and Technology
Medical Cell BioPhysics (MCBP)
Building: Carré
Room: CR4433

Drienerlolaan 5
P.O. Box 217
7500 AE Enschede
The Netherlands

Project background

Genetic and phenotypic characterization of Circulating Tumor Cells (CTC) offers the opportunity for a “real time liquid biopsy”. Heterogeneity and rarity of CTC command the need for individual cell characterization. Following an enrichment procedure of CTC from blood, the identification, isolation and manipulation of single cells for further analysis without cell loss remains challenging. Microfluidic device is a powerful tool to improve genetic and phenotypic analysis of single cells by making use of the smaller reagent volume, automation and parallel reaction.

Project goal

Develop and optimize microfluidic devices for single cancer cell characterization such as single cell isolation, lysis, DNA amplification, and drug response monitoring.

Figure 1
.(Left) Real photo of a microfluidic device for single cell whole genome amplification (Right) Schematic view. Single cell was isolated, lysed, and their DNA were amplified on a chip. Amplified DNA were validated using qPCR targeting template-specific genes such as ERBB2. PLoS ONE 9(9): e107958. doi:10.1371/journal.pone.0107958

Figure 2.
Schematic view of a microfluidic device with open-well structure for single cell whole genome amplification following filtration

Figure 3.
Setup for operation of pneumatic microfluidic device