studproj025

Fabricating Magnetic Nanoparticles for Manipulation of Cell Membrane

Bachelor’s / Saxion assignment; project will be tailored depending on duration and interests of the student

Introduction

Superparamagnetic iron oxide nanoparticles (SPIONs) find use in numerous biomedical applications including tumor imaging, drug delivery and magnetically induced hyperthermia due to their small size, biocompatibility and magnetic content.

This project involves fabricating 10-30nm antibody functionalized iron oxide nanoparticles. These particles will be used as probes of electromagnetic tweezer setup present in our lab, in order to control aggregation states of cell receptors. Particles will be fabricated by chemical co-precipitation method, coated with polymers and functionalized with antibodies.

Figure 1: Illustration of cell membrane receptor manipulation by nanoparticles: (I) SPIONs are bound to cell membrane receptors via antibodies (II) Homogeneous magnetic field is applied to SPIONs that results in chain formation of particles, sending possible activation signals to cells.

This project is a part an ongoing PhD project in Nanobiophysics in collaboration with Tumor Immunology Lab from Radboud University Nijmegen Medical Centre. The bachelor’s project will be carried out also in collaboration with MIRA NeuroImaging Group.

You will

-

Fabricate nanoparticles and functionalize them with antibodies

-

Characterize size distribution and magnetic content of magnetic nanoparticles

-

Magnetically manipulate particles into forming chain structures, image these structures and compare with available simulation software. If time allows, test functionality using T-cells and read out with cell activation indicators.

You will learn

-

Skills: Synthesizing nanoparticles via bottom up approaches and their functionalization

-

Equipment: Confocal fluorescence microscopy, dynamic light scattering, transmission electron microscopy, vibrating sample magnetometer.

Contact details

For more information you are encouraged to contact:

Burcu Çelikkol
E-mail:
b.celikkol@utwente.nl
Room: Zuidhorst ZH 165