Ultrasensitive DNA detection using silver nanoparticles


At the Nanobiophysics group we have developed a sensing device with near single recognition event sensitivity based on plasmonic interactions between gold nanoparticles. We have recently shown that this approach can detect DNA at sub-picomolar concentrations without the need for PCR amplification (see http://dx.doi.org/10.1016/j.bios.2011.06.019).

Although gold nanoparticles have a large scattering cross-section, silver nanoparticles have an even larger scattering cross-section (for the same size). Therefore, implementation of silver nanoparticles in the sensing device is predicted to increase the signal to noise ratio and therefore also increase the sensitivity.


In this project you will create DNA-modified silver particles, and compare their stability and sensitivity in our sensing device. Depending on your background, some numerical simulation on the plasmon interaction between the gold and silver nanoparticles will also be done.

Description: assay-sandwich

Basic scheme of ultrasensitive DNA detection based on plasmonic coupling of gold nanoparticles



Contact details: Dr. Ron Gill, r.gill@utwente.nl 053-4893067, ZH168.(Nanobiophysics group)