In 2005 Volodymyr Shvadchak graduated from Kyiv National University (Ukraine) holding a diploma in organic chemistry and started PhD studies in Strasbourg University (France) in biophysical lab of Yves Mely. His work was focused on development of environment-sensitive fluorescent labels and application of them in monitoring protein interactions. He defined his PhD in 2009 and started to work as a postdoc in the group of Tom Jovin in Max Planck Institute for Biophysical Chemistry (Göttingen, Germany) studying the membrane binding of α-synuclein. He joined the Nanobiophysics group in September 2012 as a postdoctoral fellow and continue studies of α-synuclein membrane interactions focusing on the properties of protein oligomers.
-Contact Information -
V. Shvadchak (Dr)
University of Twente
7522 NB Enschede, the Netherlands
7500AE Enschede, the Netherlands
TOPIC : Interaction of α-Synuclein oligomers with membranes
α-synuclein is an intrinsically disorderd protein that is deposited in form of amyloid fibrils in brain during Parkinson Disease. This protein can form oligomers and disrupt integrity of cellular membranes. I am studying the structure of α-synuclein oligomers in solution and in membrane bound form using fluorescence of environment-sensitive labels in order to understand the mechanism of α-synuclein-induced membrane leakage and to check the pore formation hypothesis.
I am also interested in the determination of the conformation of monomeric α-synuclein on membrane and in monitoring of the α-synuclein oligomerization on membrane using 2D FCS technique.
Publications of interest
Shvadchak, V.V., Yushchenko, D.A., Pievo, R. and Jovin, T.M. (2011) The mode of alpha-synuclein binding to membranes depends on lipid composition and lipid to protein ratio. FEBS Lett (Text)
Shvadchak, V.V., Falomir-Lockhart, L.J., Yushchenko, D.A. and Jovin, T.M. (2011) Specificity and kinetics of alpha-synuclein binding to model membranes determined with fluorescent excited state intramolecular proton transfer (ESIPT) probe. J Biol Chem, 286, 13023-13032 (Text)
Shvadchak, V.V., Falomir-Lockhart, L.J., Yushchenko, D.A. and Jovin, T.M. (2011) Interactions of α-Synuclein with Lipids and Artificial Membranes Monitored by ESIPT Probes, in Lipids and Cellular Membranes in Amyloid Diseases (ed R. Jelinek), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, Germany. doi: 10.1002/9783527634323.ch1 (Text)
Environment-sensitive peptide labels
Postupalenko, V. Y., Shvadchak, V. V., Duportail, G., Pivovarenko, V. G., Klymchenko, A. S., Mely, Y. (2011). Monitoring membrane binding and insertion of peptides by two-color fluorescent label. Biochim Biophys Acta 1808, 424-32 (Text)
Shvadchak, V.V., Klymchenko, A.S., de Rocquigny, H. and Mely, Y. (2009) Sensing peptide–oligonucleotide interactions by a two-color fluorescence label: application to the HIV-1 nucleocapsid protein. Nucleic Acids Res , 37, (3), e25. (Text)