Ramesh Subramani obtained his bachelor degree in General Chemistry, and then he continued his Master degree in Applied Chemistry at National Institute of Technology in India. After finishing his Masters in 2007, he joined as a research assistant at Aarhus University, Denmark in Bio-SPM group for a short period. Followed by that, he started his PhD in the same group at Aarhus University from 2008 to April 2011. His PhD degree mainly focused on ‘‘Scanning Probe Microcopy studies on DNA nanostructures’’ to develop the functional materials. After obtaining the PhD degree, he moved to Twente University at Nanobiophysics group for his post doc. research and now he is investigating the mechanical properties of amyloid fibrillar using Atomic Force microscopy.
Apart from Science, his main hobbies are sports- particularly cricket and traveling.
R. Subramani (PhD)
MESA+ Institute for Nanotechnology
University of Twente
7522 NB Enschede, the Netherlands
7500AE Enschede, the Netherlands
-Own Research in NBP
Topic: Nanomechanical characterization of supramolecular protein structures using atomic force microscopy
Many peptides and proteins have been shown to have a tendency to aggregate into ordered fibrillar nanostructures both in vitro and in vivo. Interestingly, little is known about the structure of these proteins within the fibril, and about the interactions between individual protein monomers that drive the aggregation process. The main objective of this project is to gain a better fundamental understanding of (i) the molecular properties of the individual protein molecule and the structural and mechanical properties of the resulting fibrillar structures and (ii) the properties of these fibrillar structures and the mechanical, structural and rheological properties of the solution of entangled, semi-flexible fibers on a mesoscopic scale. To achieve this, atomic force microscopy imaging, nanomechanical bending and nano-indentation on individual fibrillar protein aggregates and on the mesoscopic gels they form, are used.
Publications of interest
1. Subramani R, Juul S, Rotaru A, Andersen F F, Gothelf K V, Mamdouh W,Besenbacher F, Dong M, Knudsen B R. ‘A Novel Secondary DNA Binding Site in Human Topoisomerase I Unravelled by using a 2D DNA Origami Platform’, Accepted to ACS nano. ODI: 10.1021/nn101662a
2.M. A. Habeeb Muhammed, S. Ramesh, S. S. Sinha, S. K. Pal and T. Pradeep. ‘Two Distinct Fluorescent Quantum Clusters of Gold Starting from Metallic Nanoparticles by pH-Dependent Ligand Etching’, Nano Res., 1 (2008) pp.333-340.
3.Voigt N V, Tørring T, Rotaru A, Jacobsen M F, Ravnsbæk J B, Subramani R,Mamdouh W, Kjems J, Mokhir A, Besenbacher F, Gothelf K V. 'Single-molecule chemical reactions on DNA origami', Nature Nanotechnology, vol. 5 (2010), pp. 200-203.
4.Andersen E S, Dong M, Nielsen M M, Jahn K, Subramani R, Mamdouh W, Golas M.M, Sander B, Stark H, Oliveira C L P, Pedersen J S, Birkedal V, Besenbacher F, Gothelf K V, Kjems J, 'Self-assembly of a nanoscale DNA box with a controllable lid', Nature, vol. 459 (2009) no. 7243, pp. 73-6
5.Bald I, Weigelt S, Ma X, Xie P, Subramani R, Dong M, Wang C, Mamdouh W, Wang J, Besenbacher F. 'Two-dimensional network stability of nucleobases and amino acids on graphite under ambient conditions: Adenine, L-serine and L-tyrosine', Physical Chemistry Chemical Physics, vol. 12 (2010), pp. 3616-3621.