Martin Bennink

Martin L. Bennink

Assistant professor Nanobiophysics

University of Twente

Institute for Nanotechnology MESA+

Email: m.l.bennink@utwente.nl

Phone: + 31 53 489 6800

Visiting address:

Drienerlolaan 5

Building Zuidhorst ZH155

7522 NB  Enschede, the Netherlands

Postal address:

PO-box 217

7500 AE Enschede, the Netherlands

Research activities:

Nanomechanical characterization of biomaterials

My research work is focused on developing and exploiting methods for mechanical characterization of biomaterials at the nanometer (and larger) scale. Techniques that are used are atomic force microscopy and optical tweezers.

One particular biomaterial I am studying are protein amyloid fibrils. Atomic force microscopy imaging and mechanically characterizing alpha-synuclein fibers helps in unraveling its structure, i.e. the way that individual protein molecules are assembled to form the fibril. Measuring the mechanical properties at different length scales allows you to get a glimpse on how these mechanical properties of the individual molecules contribute through their hierarchicial structure to those of the larger structure. To this end we are attempting to perform bending tests on individual protein fibers using the atomic force microscope on one hand and perform nano-indentation experiments both on fibrils as well as on gels that are made from these fibers.

Another biomaterial of interest is collagen. We have been pioneers in developing micromechanical tensile test and bending experiments using atomic force microscopy in order to measure collagen fibrils. At the moment we are looking into the mechanical properties of individual collagen molecules, using AFM imaging as well as AFM-based single molecule force spectroscopy. By studying these and the mechanical properties of larger aggregates, such as the collagen fibril, we expect to get a better understanding of collagen works within our body.

Other activities involve the optical tweezers as a tool to perform force spectroscopy on single molecules, ranging from DNA, DNA-protein complexes and in the future hopefully towards stretching individual protein molecules.

Key words: atomic force microscopy, optical tweezers, amyloid fibers, collagen, force spectroscopy

Publications:

Sweers, K.K.M., Stöckl, M., Bennink, M.L., Subramaniam, V., Characterizing Nanoscale Morphologic and Mechanical Properties of α-Synuclein Amyloid Fibrils with Atomic Force Microscopy, in: Bio-nanoimaging: Protein Misfolding and Aggregation, November 2013, 309-322 (2013)

Sweers, K.K.M., Van Der Werf, K.O., Bennink, M.L., Subramaniam, V., Atomic force microscopy under controlled conditions reveals structure of c-terminal region of α-synuclein in amyloid fibrils, ACS Nano 6, 5952-5960 (2012)

Sweers, K.K.M., Segers-Nolten, I.M.J., Bennink, M.L., Subramaniam, V., Structural model for α-synuclein fibrils derived from high resolution imaging and nanomechanical studies using atomic force microscopy, Soft Matter 8, 7215-7222 (2012)

Sweers, K.K.M., Bennink, M.L., Subramaniam, V., Nanomechanical properties of single amyloid fibrils, Journal of Physics Condensed Matter 24, 243101 (2012).

Sweers, K.K.M., Van Der Werf, K.O., Bennink, M.L., Subramaniam, V., Spatially resolved frequency-dependent elasticity measured with pulsed force microscopy and nanoindentation, Nanoscale 4, 2072-2077 (2012)

Yang, L., van der Werf, K.O., Dijkstra, P.J., Feijen, J., Bennink, M.L., Micromechanical analysis of native and cross-linked collagen type I fibrils supports the existence of microfibrils, Journal of the Mechanical Behavior of Biomedical Materials 6, 148-158 (2012)

Croisier, F., Duwez, A.-S., Jérôme, C., Léonard, A.F., Van Der Werf, K.O., Dijkstra, P.J., Bennink, M.L., Mechanical testing of electrospun PCL fibers, Acta Biomaterialia 8, 218-224 (2012)

Murade, C.U., Subramaniam, V., Otto, C., Bennink, M.L., Single-molecule DNA force spectroscopy to probe interactions with the tri-peptide lys-trp-lys, ChemPhysChem 12, 2545-2548 (2011)

Murade, C.U., Subramaniam, V., Otto, C., Bennink, M.L, Force spectroscopy and fluorescence microscopy of dsDNA-YOYO-1 complexes: Implications for the structure of dsDNA in the overstretching region, Nucleic Acids Research 38, 3423-3431 (2010)

Murade, C.U., Subramaniam, V., Otto, C., Bennink, M.L., Interaction of oxazole yellow dyes with DNA studied with hybrid optical tweezers and fluorescence microscopy, Biophysical Journal 97, 835-843 (2009)

Bussiek, M., Hoischen, C., Diekmann, S., Bennink, M.L., Sequence-specific physical properties of African green monkey alpha-satellite DNA contribute to centromeric heterochromatin formation, Journal of Structural Biology 167, 36-46 (2009)

Gadgil, V.J., Tong, H.D., Cesa, Y., Bennink, M.L., Fabrication of nano structures in thin membranes with focused ion beam technology, Surface and Coatings Technology 203, 2436-2441 (2009)

Koenders, M.M.J.F., Yang, L., Wismans, R.G., van der Werf, K.O., Reinhardt, D.P., Daamen, W., Bennink, M.L., Dijkstra, P.J., van Kuppevelt, T.H., Feijen, J., Microscale mechanical properties of single elastic fibers: The role of fibrillin-microfibrils, Biomaterials 30, 2425-2432 (2009)

Yang, L., Van Der Werf, K.O., Fitié, C.F.C., Bennink, M.L., Dijkstra, P.J., Feijen, J., Mechanical properties of native and cross-Linked type I collagen fibrils, Biophysical Journal 94, 2204-2211 (2008)

Yang, L., Fitié, C.F.C., van der Werf, K.O., Bennink, M.L., Dijkstra, P.J., Feijen, J., Mechanical properties of single electrospun collagen type I fibers, Biomaterials 29, 955-962 (2008)

Yang, L., Van Der Werf, K.O., Koopman, B.F.J.M., Subramaniam, V., Bennink, M.L., Dijkstra, P.J., Feijen, J., Micromechanical bending of single collagen fibrils using atomic force microscopy, Journal of Biomedical Materials Research - Part A 82, 160-168 (2007)

Huisstede, J.H.G., Subramaniam, V., Bennink, M.L., Combining optical tweezers and scanning probe microscopy to study DNA-protein interactions, Microscopy Research and Technique 70, 26-33 (2007)

Van Der Rijt, J.A.J., Van Der Werf, K.O., Bennink, M.L., Dijkstra, P.J., Feijen, J., Micromechanical testing of individual collagen fibrils, Macromolecular Bioscience 6, 699-702 (2006)

Huisstede, J.H.G., Van Rooijen, B.D., Van Der Werf, K.O., Bennink, M.L., Subramaniam, V., Dependence of silicon position-detector bandwidth on wavelength, power, and bias, Optics Letters 31, 610-612 (2006)

Huisstede, J.H.G., Van Der Werf, K.O., Bennink, M.L., Subramaniam, V., Force constant calibration corrections for silicon position detectors in the near-infrared, Optics Express 14, 8476-8481 (2006)

Pope, L.H., Bennink, M.L., Van Leijenhorst-Groener, K.A., Nikova, D., Greve, J., Marko, J.F., Single chromatin fiber stretching reveals physically distinct populations of disassembly events, Biophysical Journal 88, 3572-3583 (2005)

Huisstede, J.H.G., Van Der Werf, K.O., Bennink, M.L., Subramaniam, V., Force detection in optical tweezers using backscattered light, Optics Express 13, 1113-1123 (2005)

Nikova, D.N., Pope, L.H., Bennink, M.L., Van Leijenhorst-Groener, K.A., Van Der Werf, K., Greve, J., Unexpected binding motifs for subnucleosomal particles revealed by atomic force microscopy, Biophysical Journal 87, 4135-4145 (2004)

Kassies, R., Van Der Werf, K.O., Bennink, M.L., Otto, C., Removing interference and optical feedback artifacts in atomic force microscopy measurements by application of high frequency laser current modulation, Review of Scientific Instruments 75, 689-693 (2004)

Leuba, S.H., Bennink, M.L., Zlatanova, J., Single-Molecule Analysis of Chromatin, Methods in Enzymology 376, 73-105 (2004)

Uricanu, V.I., Duits, M.H.G., Nelissen, R.M.F., Bennink, M.L., Mellema, J., Local structure and elasticity of soft gelatin gels studied with atomic force microscopy, Langmuir 19, 8182-8194 (2003)

Bennink, M.L., Nikova, D.N., Van der Werf, K.O., Greve, J., Dynamic imaging of single DNA-protein interactions using atomic force microscopy, Analytica Chimica Acta 479, 3-15 (2003)

Pope, L.H. , Bennink, M.L., Greve, J., Optical tweezers stretching of chromatin, Journal of Muscle Research and Cell Motility 23, 397-407 (2002)

Bennink, M.L., Pope, L.H., Leuba, S.H., De Grooth, B.G., Greve, J., Single chromatin fibre assembly using optical tweezers, Single Molecules 2, 91-97 (2001)

Bennink, M.L., Leuba, S.H., Leno, G.H., Zlatanova, J., De Grooth, B.G., Greve, J., Unfolding individual nucleosomes by stretching single chromatin fibers with optical tweezers, Nature Structural Biology 8, 606-610 (2001)

Rusu, C., Oever, R.V., De Boer, M.J., Jansen, H.V., Berenschot, J.W., Bennink, M.L., Kanger, J.S., De Grooth, B.G., Elwenspoek, M., Greve, J., Brugger, J., Van Berg, A.D., Direct integration of micromachined pipettes in a flow channel for single DNA molecule study by optical tweezers, Journal of Microelectromechanical Systems 10, 238-246 (2001)

Rusu, Cristina, van't Oever, Ronny, de Boer, Meint, Jansen, Henri, Berenschot, Erwin, Elwenspoek, Miko, Bennink, Martin L., Kanger, Johannes S., de Grooth, Bart G., Greve, Jan, Brugger, Juergen, van den Berg, Albert, Micromachined pipettes integrated in a flow channel for single DNA molecule study by optical trapping, Proceedings of SPIE - The International Society for Optical Engineering, Micro- and Nanotechnology for Biomedical and Environmental Applications; San Jose, CA, USA; ; 26 January 2000 through 27 January 2000, 41-49 (2000)

Rusu, Cristina, van't Oever, Ronny, de Boer, Meint, Jansen, Henri, Berenschot, Erwin, Bennink, M.L., Kanger, J.S., de Grooth, B.G., Elwenspoek, Miko, Greve, J., van den Berg, A., Brugger, J., Fabrication of micromachined pipettes in a flow channel for single molecule handling of DNA, Proceedings of the IEEE Micro Electro Mechanical Systems (MEMS), 13th Annual International Conference on Micro Electro Mechanical Systems (MEMS 2000); Miyazaki, Jpn; ; 23 January 2000 through 27 January 2000, 429-434 (2000)

Bennink, M.L., Schärer, O.D., Kanaar, R., Sakata-Sogawa, K., Schins, J.M., Kanger, J.S., De Grooth, B.G., Greve, J. Single-molecule manipulation of double-stranded DNA using optical tweezers: Interaction studies of DNA with RecA and YOYO-1, Cytometry 36, 200-208 (1999)