Kristian Göeken

Kristian finished his study biomedical technology at the University of Twente in 2008. He did his bachelor assignment at the Polymer Chemistry and Biomaterials group (PBM) where he studied the stability and transfection efficiency of nano-sized polymeric gene carriers. Afterwards he continued on at the UT with the master Biomedical Engineering, following the track Molecular, Cellular & Tissue Engineering. He did an internship at Innocore Technologies in Groningen, where he studied the effect of polysaccharides on the formation, stability and drug delivery properties of protein encapsulated microspheres. In the summer of 2011 he finished his Master thesis in the Tissue Regeneration group, where he studied the effects of dexamethasone release out of electrospun scaffolds, with the purpose of enhancing growth and osteogenic differentiation of human mesenchymal stem cells.

In January 2012 he joined the Nanobiophysics group (NBP) where he is currently doing research on the topic of detecting bacterial DNA using Localized Surface Plasmon Resonance (LSPR) of oligonucleotide functionalized gold nanoparticles.

-Contact Information -

Kristian Göeken (MSc)

Nanobiophysics group

University of Twente

Institute for Nanotechnology MESA+

Zuidhorst ZH167

Drienerlolaan 5

7522 NB  Enschede, the Netherlands

PO-box 217

7500AE Enschede, the Netherlands

T +31-(0)53-489-4612

TOPIC: Sensing of bacterial DNA using Localized Plasmon Resonance (LSPR) of oligonucleotide functionalized gold nanoparticles

Localized Plasmon Resonance (LSPR) is an optical phenomenon which is generated when light hits nanoparticles made out of conductive materials such as gold or silver. Depending on the material, size and geometry of the particle and its immediate dielectric environment, light at a certain wavelength causes the electrons of the conduction band to oscillate at its plasmon frequency. At this frequency, the nanoparticles exhibit enhanced absorption and scattering as well as electromagnetic near-field enhancement. Changes in the dielectric environment (such as absorbed molecules on the surface of the particle) causes the plasmon frequency to shift, which in turn shifts the absorption bands and scattered intensity. This effect can be exploited as a sensing method.

In my project, (gold) nanoparticles are functionalized with single stranded DNA (ssDNA), which can hybridize with a part of a target strand (such as DNA from mycobacterium tuberculosis). A second set of nanoparticles is then deposited on a surface and functionalized with ssDNA, which can also hybridize part of the target DNA strand. When the target DNA strand is subsequently introduced, hybridization occurs, which pulls two nanoparticles close together. Due to the proximity of both particles, the LSPR effect is enhanced and the plasmon frequency shifts, making it possible to detect (single) molecule binding events (e.g. using Dark Field microscopy).

The aim of my project is to use this detection scheme to develop an efficient, sensitive, specific and easy to use microfluidic sensor capable of sensing bacterial DNA.

Publications of interest

Hurst, S.J., Lytton-Jean, A.K.R., Mirkin, C.A. Maximizing DNA loading on a range of gold nanoparticle sizes. (2006) Analytical Chemistry, 78 (24), pp. 8313-8318

Verdoold, R., Gill, R., Ungureanu, F., Molenaar, R., Kooyman, R.P.H. Femtomolar DNA detection by parallel colorimetric darkfield microscopy of functionalized gold nanoparticles (2011) Biosensors and Bioelectronics, 27 (1), pp. 77-81.