A graduate (2006) in Applied Sciences from University of Allahabad, India, and a post graduate (2008) in Biotechnology from School of Biotechnology (SBT) Jawaharlal Nehru University (JNU), New Delhi, India. He acquired sound training in basic biotechnology techniques and the intricacies of research through the one year dissertation titled “Studies on the effect of some amino acids on the insulin amyloid formation”, under the supervision of Prof. Rajiv Bhat, Biophysical Chemistry Lab, SBT, JNU. The major impetus of the work was to instill adeptness in technicalities and challenges associated with understanding protein aggregation and how solvent mediated effects on protein stability could possibly influence amyloidogenic tendency of the protein. Subsequent to successful completion of his masters in 2008, he joined National Institute of Immunology (NII) New Delhi, India as a Junior Research Fellow, working with Dr Monica Sundd, Scientist IV, Bimolecular NMR facility. Working on project titled, “Investigation of the structural organization of B-cell linker protein and the role of its domains in the formation of signalosome”, In parallel he was working on the recombinant production of A β(1-42) peptide. He gained appreciable experience in recombinant DNA techniques and basics of Biomolecular Nuclear Magnetic Resonance spectroscopy. He is currently pursuing his doctorate at University of Twente, Nanobiophysics group, studying the finer aspects of structural biology and biophysics of proteins during amyloidogenesis in a confined environment.
Beyond academics, Himanshu is an avid sociophile looking forward to communicate with people from diverse walks of life to address any scientifically or socially critical issue. He has been a regular participator in Science Day Celebrations at the University of Allahabad and has served as a member of the Jawaharlal Nehru University Students’ Union Election Committee (2006-07) including as a Councillor of School of Biotechnology, Jawaharlal Nehru University. Presently, he is a member of the Social organisation “AADHAAR” in The Netherlands aimed to help rural people of India.
Himanshu Chaudhary (MSc)
University of Twente
Institute for Nanotechnology MESA+
7522 NB Enschede, The Netherlands
7500AE Enschede, The Netherlands
Research in NBP
TOPIC:Amyloids in confinement
In neurodegenerative diseases the self assembly of proteins into fibrils and of fibrils into mesoscopic aggregates does not take place in a large volume. Amyloid self assembles in spaces of sub cellular dimensions which makes the average test tube a bad model system. Additionally the aggregation does not take place in simple solutions. In brain cells membrane surfaces or other proteins may interact with the amyloidogenic proteins or amyloid fibrils. The influence of all these factors makes it difficult to understand the biophysics of amyloid aggregation in vivo. In this project we will try to mimic aspects of the complex environment of the cell and follow amyloid fibril self assembly in (membrane enclosed) compartments of cellular dimensions using micro patterning, optical microscopy and spectroscopy techniques.
(funded by NWO-CW Vidi*)
Lipid matrix induced aggregates of α synuclein ,Imaged by 20X objective NIKON 2000