harmen_mulder

Short Biography

After finishing his VWO at Piter Jelles Montessori in Leeuwarden, Harmen started his study Applied Physics at the University of Twente in 2004. During his bachelor he followed some courses of the minor Biomedical Engineering. Here, his interest in the biology started to grow. For that reason, Harmen decided to start a master in Optics and Biophysics. For this master he did an internship at the hospital in Enschede (Medisch Spectrum Twente). The study in optimizing the administered FDG-dose for PET imaging resulted in some nice results which were published (see publications of interest, internship) and implemented in the hospital. This really motivated him to continue in the research field of Biophysics and he started his master thesis at Nanobiophysics group (NBP) at the University of Twente. During the master thesis he worked on the design, realization and characterization of a combined Total Internal Reflection Fluorescence (TIRF) and Fluorescence Photoactivation Localization Microscopy (FPALM) Super-resolution microscope. In January 2011 he started his PhD in the Nanobiophysics group on Multiple Wavelength Integrated Young Interferometer for Ultra-Sensitive Sensing (see below). During his PhD he also got his master degree in Science Education and Communication.

Next to his work, Harmen was a member of the P-NUT board. P-NUT is an organization that represents all the PhD’s on the University of Twente. His function in the PNUT board was treasurer. He worked also as a treasurer at BSV De Stoottroepen, the snooker, pool and billiards association at the University of Twente. Playing futsal, squash and tennis are his hobbies. Besides, he watches sports on TV, especially cycle racing, football and tennis.

Contact Information

H.K.P. Mulder (MSc)

Nanobiophysics

Zuidhorst ZH166

Drienerlolaan 5

7522 NB  Enschede, the Netherlands

P +31-(0)53-489-3466

h.k.p.mulder(at)utwente.nl

Own Research in NBP

TOPIC: Multiple Wavelength Integrated Young Interferometer for Ultra-Sensitive Sensing

With an integrated optical Young interferometer sensor we can very sensitively detect refractive index changes in the evanescent field above the waveguide core. In this way it is possible to detect an analyte concentration in a given sample solution, such as virus particles and proteins. Although this method is very sensitive, certain applications of this sensor are hampered by limited specificity. Refractive index changes due to non-specific binding or due to bulk changes cannot be discriminated from the refractive index changes due to the process where you are interested in. To overcome this problem, the idea is to use an alternative method, measuring simultaneously with multiple wavelengths (see figure 2). The theoretical possibilities of this new method, called Size-selective detection, were investigated (see publications PhD). Furthermore, proof-of-principle measurements were performed illustrating the addition of size-selectivity to the YI sensor using multiple wavelength and will hopefully be published soon.

Fig.1: Schematic overview of an integrated Young interferometer immunosensor.

Fig.2: Waveguide structure with mode profiles of three different wavelengths to discriminate between specific binding, non-specific binding and refractive index change in solvent which is covering the waveguide.

Output

PhD:

Publications:

1.

Mulder, H.K.P., Ymeti, A., Subramaniam, V. & Kanger, J.S. (2012). Size-selective detection in integrated optical interferometric biosensors. Optics Express, 20(19), 20934-20950.

Oral presentations:

1.

Mulder, H.K.P., Kanger, J.S. (2011, June 16). Multiple wavelength integrated Young interferometer for ultra-sensitive biosensing, Applied Nano Photonics retreat, Ootmarsum, The Netherlands.

2.

Mulder, H.K.P., Subramaniam, V., Kanger, J.S. (2013, March). Size-selective detection in an integrated optical Young interferometer biosensor. 2nd meeting for NanoNextNL programme 03B, Boekelo, The Netherlands.

3.

Mulder, H.K.P., Kanger, J.S. (2012, October 2). Size-selective detection in integrated optical interferometric biosensors. Annual Dutch meeting on Molecular and Cellular Biophysics, Veldhoven, The Netherlands.

4.

Mulder, H.K.P., Ymeti, A., Dudia, A., Subramaniam, V. & Kanger, J.S. (2012, December 10). Size-Selective Detection in Integrated Optical Interferometric Biosensors. MicroNanoConference'12, Ede, The Netherlands.

5.

Mulder, H.K.P., Subramaniam, V., Kanger, J.S. (2013, January). Size-selective detection in an integrated optical Young interferometer biosensor. MIRA Imaging & Diagnostics colloquium, Enschede, The Netherlands.

6.

Mulder, H.K.P., Subramaniam, V., Kanger, J.S. (2013, March). Size-selective detection in an integrated optical Young interferometer biosensor. ANP Meeting 03B, Enschede, The Netherlands.

7.

Mulder, H.K.P., Subramaniam, V., Kanger, J.S. (2013, June). Size-selective detection in an integrated optical Young interferometer biosensor. 3rd meeting for NanoNextNL programme 03B, Utrecht, The Netherlands.

8.

Mulder, H.K.P., Subramaniam, V., Kanger, J.S. (2013, September 16). Size-selective detection in an integrated optical Young interferometer biosensor. MESA+ dag: Enschede, The Netherlands.

9.

Mulder, H.K.P., Subramaniam, V., Kanger, J.S., (2014, April 24) Size-selective detection in an integrated optical Young interferometer. 4th meeting NanoNextNL programme 03B, Utrecht, The Netherlands.

10.

Mulder, H.K.P., Subramaniam, V., Kanger, J.S. (2014, July 31), Background Reduction in a Young Interferometer Biosensor, Advanced Photonics Congress, Barcelona, Spain.

11.

Mulder, H.K.P., Subramaniam, V. & Kanger, J.S. (2014, September 9). Improving the specificity of an integrated optical Young interferometer biosensor. MIRA Imaging and Diagnostics colloquium, Enschede, The Netherlands.

12.

Mulder, H.K.P., Subramaniam, V. & Kanger, J.S. (2014, September ). Size-selective detection in an integrated optical Young interferometer. NanoNext NL Program 10 Theme meeting Sensors and Actuators, Utrecht,The Netherlands.

13.

Mulder, H.K.P., Subramaniam, V. & Kanger, J.S. (2014, October 27). Background Reduction in a Young Interferometer Biosensor. NanoCity 2014 Utrecht, The Netherlands.

Poster presentations:

1.

Mulder, H.K.P., Ymeti, A., Subramaniam, V. & Kanger, J.S. (2011, October 03-04). Development of a multi-wavelength integrated Young interferometer biosensor. Annual Dutch meeting on Molecular and Cellular Biophysics: Veldhoven, The Netherlands.

2.

Mulder, H.K.P., Ymeti, A., Dudia, A., Subramaniam, V. & Kanger, J.S. (2012, May 15 18). Development of a Multi-Wavelength Integrated Young Interferometer Biosensor. Biosensors 2012: Cancun, Mexico.

3.

Mulder, H.K.P., Ymeti, A., Dudia, A., Subramaniam, V. & Kanger, J.S. (2012, November 01-03). Size-Selective Detection in an Integrated Young interferometer Biosensor. Label-free technologies: Amsterdam, The Netherlands.

4.

Mulder, H.K.P., Subramaniam, V. & Kanger, J.S. (2013, November). Size-selective Detection in an Integrated Optical Young Interferometer Biosensor. NanoNexNL NanoMedicine Day, Amsterdam, The Netherlands.

5.

Mulder, H.K.P., Subramaniam, V. & Kanger, J.S. (2013, November). Size-Selective Detection in an Integrated Optical Young Interferometer Biosensor. MicroNanoConference ’13, Ede, The Netherlands.

6.

Mulder, H.K.P., Subramaniam, V. & Kanger, J.S. (2014, oktober 27). Size-selective Detection in an Integrated Optical Young Interferometer Biosensor. NanoCity 2014, Utrecht, The Netherlands.

Internship:

Publications:

1.

Dr. J.A. van Dalen, Dhr. H. Mulder, Ir. P. Kamp, Drs. W. de Bruin, Drs. M.D. Zuijdwijk, Prof. Dr. V. Subramaniam, Optimizing administered FDG dose for PET imaging, Tijdschrift voor Nucleaire Geneeskunde 2010; 32(1): 433-437.

a.

Presented at yearly NVKF congress in Woudschoten, April 9th and 10th 2010

b.

Presented at 10th congress of the “World Federation of Nuclear Medicine and Biology (WFNMB)”, September 18th- 23rd 2010, abstract published in World Journal of Nuclear Medicine, Volume 9, Suppl. 1, Sept. 2010, pp S-8

c.

Presented at yearly congress of “European Association of Nuclear Medicine (EANM)”, October 9th-13th 2010, abstract published in European Journal of Nuclear Medicine & Molecular Imaging, Volume 37, Suppl. 2, Oct. 2010, pp S-262.