dorothee_wasserberg

-Short Biography-

Dorothee Wasserberg graduated in Chemistry from the Friedrich-Alexander-University, Erlangen-Nürnberg, Germany, and the Technical University Eindhoven, Netherlands, in 2002. She received her Ph.D. degree from the Technical University Eindhoven, Netherlands, in 2006 after 4 years of research on phosphorescence and triplet energy transfer in functional molecules with prof. dr. ir. R.A.J. Janssen. She spent the next two years at the Chemical Genomics Center of the Max-Planck-Institute for Molecular Physiology, Dortmund, Germany, with prof. dr. ir. L. Brunsveld, studying protein-protein interactions using immobilized non-natural cofactor-mimics as a postdoctoral Max-Planck fellow, after which she joined the group of prof. dr. V. Subramaniam and dr. R.P.H. Kooyman at the University of Twente, Netherlands, in 2008 where she conducted research on the construction and characterization of gold nanoparticle immuno sensor arrays. In 2009 she started her own research project as a DAAD postdoctoral fellow of the German Academic Exchange Service on the orientational control and spectroscopic characterization of immobilized (fluorescent) proteins in the groups of prof. dr. V. Subramaniam of prof. dr. J. Huskens and dr. P. Jonkheijm at the University of Twente, Netherlands.

-Contact Information -

Dr. Dorothee Wasserberg

Nanobiophysics and Molecular Nanofabrication

University of Twente

Institute for Nanotechnology, MESA+

Zuidhorst ZH1.66

P.O.box 217

7500 AE Enschede, The Netherlands

P +31-(0)53-489-3466

F +31-(0)53-489-2150


d.wasserberg<at>utwente.nl

-Own Research in NBP-

TOPIC: Multiplexed Protein Nanoarrays: Full Control over Protein Orientation Localisation and Functionality

Precise control over protein orientation and geometry is crucial to the construction of uniform functional protein arrays. However, to date, no systematic study on the influence of full control over orientation and geometry of immobilisation of proteins in arrays on their functionality has been carried out.

The aim of this project is to develop methods to construct such arrays in which the effect of different degrees of control over the immobilisation geometry of proteins on their function can be studied at the surface. The immobilisation geometry of proteins on the surface will be studied with respect to their exact positioning such as localisation, orientation and number of binding sites. This will be done by studying the immobilisation geometry of proteins on substrates with differing degrees of freedom of translational and rotational movement.

Publications of interest

1.

Jonkheijm, P.; Weinrich, D.; Köhn, M.; Engelkamp, H. E.; Christianen, P. C. M.; Kuhlmann, J.; Maan, J. C.; Nüsse, D.; Schroeder, H.; Wacker, R.; Breinbauer, R.; Niemeyer, C. M.; Waldmann, H., Photochemical surface patterning by the thiol-ene reaction. Angew. Chem. Int. Ed. 2008, 47, 4421-4424.

2.

Escalante, M.; Maury, P.; Bruinink, C. M.; van der Werf, K.; Olsen, J. D.; Timney, J. A.; Huskens, J.; Hunter, C. N.; Subramaniam, V.; Otto, C., Direct assembly of functional light harvesting antenna complexes onto chemically patterned surfaces. Nanotechnology 2008, 19, (2), 025101/1-025101/6.

3.

Maury, P.; Escalante-Marun, M.; Péter, M.; Reinhoudt, D. N.; Subramaniam, V.; Huskens, J., Creating nanopatterns of His-tagged proteins on surfaces by nanoimprint lithography using specific NiNTA-histidine interactions. Small 2007, 3, (9), 1584-1592.

4.

Beulen, M. W. J.; Bugler, J.; De Jong, M. R.; Lammerink, B.; Huskens, J.; Schonherr, H.; Vancso, G. J.; Boukamp, B. A.; Wieder, H.; Offenhauser, A.; Knoll, W.; Van Veggel, F. C. J. M.; Reinhoudt, D. N., Host-guest interactions at self-assembled monolayers of cyclodextrins on gold. Chem. Eur. J. 2000, 6, (7), 1176-1183.

5.

Hutschenreiter, S.; Tinazli, A.; Model, K.; Tampe, R., Two-substrate association with the 20S proteasome at single-molecule level. EMBO J. 2004, 23, (13), 2488-2497.