Christian Blum

I studied Chemistry, specialized in physical chemistry and received my Diploma from the University of Siegen, Germany. I continued there as a PhD student in the group of Prof. A. J. Meixner and graduated in 2004 with a doctorate’s degree in natural sciences. I then joined the NBP group.

 

-Contact Information -

Christian Blum

Nanobiophysics

 

University of Twente

Institute for Nanotechnology MESA+

Zuidhorst ZH157

Drienerlolaan 5

7522 NB  Enschede, the Netherlands

PO-box 217

7500AE Enschede, the Netherlands

P +31-(0)53-489-4622

F +31-(0)53-489-2150


c.blum@utwente.nl

Research:

The steady advance of light based techniques in many areas of research, but especially in the life sciences, rests on two pillars: advancements in instrumentation and methods and the availability of entirely new classes of luminescent probes. The development of new instrumentation and methods and the use and characterization of new luminescent probes goes hand in hand. To create a maximum in synergies I am working on both these fields and directly apply new instrumentation, methods and probes to various research questions.

Imaging spectroscopy methods of various parameters enables the extension of simple microscopy to probe function, co-localization and molecular interaction. The general philosophy has been to achieve single molecule detection sensitivity, which translates to fast analysis of larger ensembles of molecules, and allows to image intensities, anisotropy, emission spectra and fluorescence lifetimes.

One focus of my single molecule work is on the characterization of fluorophores. In contrast to the traditionally used chemical dyes for fluorescence labelling, the newly developed labels like fluorescent proteins or quantum dots do not form ensembles of identical emitters, but complex, heterogeneous ensembles. The molecular properties can vary considerably from molecule to molecule and with time for individual molecules and can only be accessed by single molecule detection methods.

Further I am exploring the potential of nanophotonic manipulation and control of biological emitters – nanobiophotonics. This research on nanophotonic manipulation of biological emitters, carried out in close collaboration with the Complex Photonic Systems group of Prof. W. L. Vos, explores a range of different themes.

Research highlights – cover gallery

D:\Profiles\blumc\Desktop\PRL cover.JPGNanophotonic Control of the Foerster Resonance Energy Transfer Efficiency


C. Blum, N. Zijlstra, A. Lagendijk, M. Wubs, A.P. Mosk, V. Subramaniam, W.L. Vos
Physical Review Letters, 109, 203601 (2012)

doi: 10.1103/PhysRevLett.109.203601

Featured e.g. in:

·

Science – Editors Choice: Don’t FRET – All Under Control

·

Nature Materials – Research Highlights: Molecular energy transfer

http://www.utwente.nl/tnw/nbp/%5E%5Econtenttypesdata/2344183.jpgNon-invasive imaging through opaque scattering layers


J. Bertolotti, E.G. van Putten, C. Blum, A. Lagendijk, W.L. Vos, A.P. Mosk
Nature, 491, 232-234 (2012)

doi: 10.1038/nature11578

http://www.utwente.nl/tnw/nbp/%5E%5Econtenttypesdata/1639738.jpgMolecular Composition of Sub-stoichiometrically Labeled α-Synuclein Oligomers Determined by Single-Molecule Photobleaching


N. Zijlstra, C. Blum, I.M.J. Segers-Nolten, M.M.A.E. Claessens, V. Subramaniam
Angewandte Chemie International Edition, Volume 51, Issue 35, pages 8821–8824, August 27 (2012)

doi: 10.1002/anie.201200813

Color control of natural fluorescent proteins by photonic crystals


C. Blum, A.P. Mosk, I.S. Nikolaev, V. Subramaniam, and W.L. Vos
Small, 4(4): 492-496, (2008)

doi: 10.1002/smll.200701160

Recent publications

Full list of my publications at Google Scholar

1

Nanophotonic Control of the Foerster Resonance Energy Transfer Efficiency
C. Blum, N. Zijlstra, A. Lagendijk, M. Wubs, A. P. Mosk, V. Subramaniam, and W. L. Vos
Physical Review Letters, 109, 203601 (2012)
doi:10.1103/PhysRevLett.109.203601

2

Non-invasive imaging through opaque scattering layers
Bertolotti, J., Putten, E.G. van, Blum, C., Lagendijk, A., Vos, W.L. & Mosk, A.P.
Nature, 491, 232-234 (2012)
doi: 10.1038/nature11578

3

Patterning perylenes on surfaces using thiol–ene chemistry
D. Wasserberg,T. Steentjes, M. H. W. Stopel, J. Huskens,
C. Blum, V. Subramaniam, and P. Jonkheijm
Journal of Materials Chemistry, 22, 16606-16610 (2012)
doi:10.1039/c2jm32610h

4

Molecular Composition of Sub-stoichiometrically Labeled α-Synuclein Oligomers Determined by Single-Molecule Photobleaching
Zijlstra N, Blum C, Segers-Nolten IMJ, Claessens MMAE, Subramaniam V
Angewandte Chemie International Edition, Volume 51, Issue 35, pages 8821–8824, August 27 (2012)
doi: 10.1002/anie.201200813

5

Room temperature excitation spectroscopy of single quantum dots
Blum, C., F. Schleifenbaum, M. Stopel, S. Peter, M. Sackrow, V. Subramaniam, A. J. Meixner
Beilstein J. Nanotechnol., 2, 516-524, (2011)

6

Dark proteins disturb multichromophore coupling in tetrameric fluorescent proteins.
Blum, C., A.J. Meixner, and V. Subramaniam
Journal of Biophotonics, 4(1-2): p. 114-121 (2011)
doi:0.1002/jbio.201000075

7

Interactions of Perylene Bisimide in the One-Dimensional Channels of Zeolite L
Busby, M., A. Devaux, C. Blum, V. Subramaniam, G. Calzaferri, and L. De Cola,
Journal of Physical Chemistry C, 115(13): p. 5974-5988 (2011)
doi:10.1021/jp1108625

8

Microspectroscopic analysis of green fluorescent proteins infiltrated into mesoporous silica nanochannels.
Ma, Y.J., P. Rajendran, C. Blum, Y. Cesa, N. Gartmann, D. Bruhwiler, and V. Subramaniam,
Journal of Colloid and Interface Science, 356(1): p. 123-130 (2011)
doi:10.1016/j.jcis.2010.12.082

9

Visualizing Resonance Energy Transfer in Supramolecular Surface Patterns of β-CD-Functionalized Quantum Dot Hosts and Organic Dye Guests by Fluorescence Lifetime Imaging
Dorokhin, D., S.-H. Hsu, N. Tomczak, C. Blum, V. Subramaniam, J. Huskens, D.N. Reinhoudt, A.H. Velders, and G.J. Vancso,.
Small, 6(24): p. 2870-2876 (2010)

10

Modulation of Protein Dimerization by a Supramolecular Host-Guest System
Uhlenheuer, D.A., D. Wasserberg, H. Nguyen, L. Zhang, C. Blum, V. Subramaniam, and L. Brunsveld
Chemistry - A European Journal, 15(35): p. 8779-8790 (2009)

11

Temperature-modulated quenching of quantum dots covalently coupled to chain ends of poly(N-isopropyl acrylamide) brushes on gold.
Tagit, O., N. Tomczak, E.M. Benetti, Y. Cesa, C. Blum, V. Subramaniam, J.L. Herek, and G.J. Vancso
Nanotechnology, 20(18): p. 6; 185501 (2009)
doi:0.1088/0957-4484/20/18/185501

12

Single Molecule Spectral Dynamics at Room Temperature
Schleifenbaum, F., C. Blum, V. Subramaniam, and A.J. Meixner,.
Molecular Physics, 107(18): p. 1923-1942 (2009)
doi:10.1080/00268970802635004

13

Schleifenbaum, F., C. Blum, K. Elgass, S. Peter, V. Subramaniam, and A.J. Meixner. Multiparameter Single Molecule Spectroscopy gives insight into the complex Photophysics of Fluorescence Energy Transfer (FRET) coupled Biosystems. in Proc. of SPIE. (2009)

14

Husken, B.H., L.A. Woldering, C. Blum, and W.L. Vos, Method to deterministically study photonic nanostructures in different experimental instruments.
Journal of Microscopy-Oxford, 233(1): p. 18-23 (2009)
doi:10.1111/j.1365-2818.2008.03091.x

15

Multimode Microscopy: Spectral and Lifetime Imaging
Blum, C., Y. Cesa, M. Escalante, and V. Subramaniam,
Journal of the Royal Society Interface, 6: p. 35-43 (2009)
doi:10.1098/rsif.2008.0356.focus

16

Expression of sensitized Eu3+ luminescence at a multivalent interface
Hsu, S.H., M.D. Yilmaz, C. Blum, V. Subramaniam, D.N. Reinhoudt, A.H. Velders, and J. Huskens,.
Journal of the American Chemical Society, 131(35): p. 12567-12569 (2009)

17

FRET Pair Printing of Fluorescent Proteins
Escalante, M., C. Blum, Y. Cesa, C. Otto, and V. Subramaniam
Langmuir, 25(12): p. 7019-7024 (2009)
doi:10.1021/la900223f

18

Manipulation of the local density of photonic states to elucidate fluorescent protein emission rates
Cesa, Y., C. Blum, J.M. van den Broek, A.P. Mosk, W.L. Vos, and V. Subramaniam
Physical Chemistry Chemical Physics, 11(14): p. 2525-2531 (2009)
doi:10.1039/b817902f

19

Single-molecule spectroscopy of fluorescent proteins
Blum, C. and V. Subramaniam
Analytical and Bioanalytical Chemistry, 393(2): p. 527-541 (2009)
doi:10.1007/s00216-008-2425-x

20

Spectral emission imaging to map photonic properties below the crystal surface of 3D photonic crystals
Blum, C., A.P. Mosk, W.L. Vos, and V. Subramaniam
Journal of the Optical Society of America B, 26(11): p. 2101–2108 (2009)
doi:10.1364/JOSAB.26.002101

21

Controlling fluorescent proteins by manipulating the local density of photonic states
Blum, C., Y. Cesa, J.M. van den Broek, A.P. Mosk, W.L. Vos, and V. Subramaniam
in Proc. of SPIE. 2009. Munich, Germany: SPIE.

Book Chapters:

22

Fluorescence lifetime spectroscopy and imaging of visible fluorescent proteins
Jain, A., C. Blum, and V. Subramaniam
Advances in Biomedical Engineering, ed. P. Verdonck. Elsevier (2009)

23

Spectral versatility of Fluorescent Proteins observed on the single molecule level
Blum, C., and V. Subramaniam
Springer Series on Fluorescence, ed. G. Jung, Springer (2011)