Wu - Mesenchymal stem cells as trophic mediators in cartilage regeneration - 2012

Wu Ling

University of Twente 2012 - dissertation


Autologous Chondrocyte Implantation (ACI) is currently considered as the
golden standard for treatment of large-size cartilage defects. ACI requires, however, at
least two operative procedures which are separated by several weeks due to the
obligatory cell expansion to obtain sufficient cells for implantation. Replacement of
chondrocytes by alternative cell sources can potentially reduce the two step procedure to
a single step procedure by omitting the cell expansion phase. Hendriks et al., cocultured
bovine primary chondrocytes with human expanded chondrocytes, human
dermal fibroblasts, mouse embryonic stem cells, mouse-3T3 feeder cells, or human
mesenchymal stem cells in cell pellets [1]. Their data indicated that cartilage matrix
deposition could be supported by co-culturing chondrocytes with a variety of cell types.
In their experimental setup, the co-culture pellets contained approximately 20% of
chondrocytes, but the amount of GAG in co-culture pellets was similar to pure
chondrocytes pellets. This synergistic effect of cartilage formation in co-cultures of
chondrocytes with other cell types was defined as chondro-induction [2]. The finding of
chondro-induction potentially leads to the development of a new cell-based therapy for
cartilage regeneration: one step surgery of ACI, in which the necessity for in vitro
chondrocyte expansion in laboratory is circumvented.