Kunstar - Confocal Raman Microspectroscopy Applications in Cartilage Tissue Engineering - 2012

Kunstar Aliz,

University of Twente, dissertation 2013


Currently, advanced cartilage tissue engineering generally involves the
use of three-dimensional (3D) scaffolds, which can support the growth,
proliferation and differentiation of incorporated chondrocytes and/or
progenitor cells [1-2].
Studies performed to investigate the composition and quality of tissue
engineered cartilage are generally done by using destructive methods
like immunohistological, molecular, biochemical or microscopy
techniques. Obviously there is an emerging need for non-invasive and
non-destructive monitoring methods. These methods would allow for real
time monitoring of tissue engineered constructs and study changes in
phenontype of the cells involved and changes in extracellular matrix
deposition. This thesis aims at studying relevant problems in cartilage
tissue engineering by using confocal Raman spectroscopy which is an
excellent non-invasive and non-destructive optical tool for high resolution
spatially resolved chemical imaging and analysis of tissue engineered
samples. Moreover, unlike conventional methods which only give
information on the presence of specific compounds, Raman
spectroscopy provides a spectroscopic “fingerprint” representing the
entire molecular composition of samples of interest. In this Chapter a
general introduction is given on cartilage biology, cartilage tissue
engineering, the Raman effect, confocal Raman spectroscopy and
Raman applications in tissue engineering, more specifically in cartilage
tissue engineering.