Ma - Both - Osteogenic capacity of human BM-MSCs - AT-MSCs and their co-cultures using HUVECs in FBS and PL supplemented media

Jinling Ma, Jeroen J. J. P. van den Beucken, Sanne K. Both, Henk-Jan Prins, Marco N. Helder, Fang Yang and John A. Jansen

Journal of Tissue Engineering and Regenerative Medicine - 2015

Abstract

Human bone marrow-derived mesenchymal stem cells (BM-MSCs) and human adipose tissue-derived
mesenchymal stem cells (AT-MSCs) are the most frequently used stem cells in tissue engineering. Due
to major clinical demands, it is necessary to find an optimally safe and efficient way for large-scale
expansion of these cells. Considering the nutritional source in the culture medium and method, this
study aimed to analyze the effects of FBS- and PL-supplemented media on osteogenesis in stem cell
mono- and co-cultures with human umbilical vein endothelial cells (HUVECs). Results showed that cell
metabolic activity and proliferation increased in PL- compared to FBS-supplemented media in monoand
co-cultures for bothBM-MSCs and AT-MSCs. In addition, calciumdeposition was cell type dependent
and decreased for BM-MSCs but increased for AT-MSCs in PL-supplemented medium in both mono- and
co-cultures. Based on the effects of co-cultures, BM-MSCs/HUVECs enhanced osteogenesis compared to
BM-MSCs monocultures in both FBS- and PL-supplemented media whereas AT-MSCs/HUVECs showed
similar results compared to AT-MSCs monocultures. Copyright © 2013 John Wiley & Sons, Ltd.