Wu - Fibroblast Growth Factor-1 Is a Mesenchymal Stromal Cell-Secreted Factor Stimulating Proliferation of Osteoarthritic Chondrocytes in Co-Culture

Ling Wu, Jeroen Leijten, Clemens A. van Blitterswijk en Marcel Karperien

Stem Cells and Development 2013


Intra-articular injection of human mesenchymal stromal cells (MSCs) is currently under investigation for disease modifying treatment of osteoarthritis. In vitro experiments have shown beneficial effects of MSCs on chondrocytes in a variety of co-culture models in terms of cartilage matrix formation. We have shown that in pellet co-cultures this beneficial effect with respect of cartilaginous matrix formation is predominantly due to stimulation of chondrocyte proliferation by the MSCs rather than to chondrogenic differentiation of the MSCs themselves. In addition, we have shown that this beneficial effect on chondrocyte proliferation in pellet cultures is mainly due to the secretion of (a) soluble factor(s) by the MSCs. The objective of this study is to identify this factor(s) which may be (an) important mediator(s) of the disease modifying trophic role of MSCs in osteoarthritis.