Weerd - On-Chip Electrophoresis in Supported Lipid Bilayer Membranes Achieved Using Low Potentials

Jasper van Weerd, Sven O. Krabbenborg, Jan Eijkel, Marcel Karperien, Jurriaan Huskens and Pascal Jonkheijm

JACS - 2013

Abstract

A micro supported lipid bilayer (SLB)
electrophoresis method was developed, which functions
at low potentials and appreciable operating times. To this
end, (hydroxymethyl)-ferrocene (FcCH2OH) was employed
to provide an electrochemical reaction at the anode
and cathode at low applied potential to avoid electrolysis
of water. The addition of FcCH2OH did not alter the SLB
characteristics or affect biomolecule function, and pH and
temperature variations and bubble formation were
eliminated. Applying potentials of 0.25−1.2 V during
flow gave homogeneous electrical fields and a fast,
reversible, and strong build-up of a charged dye-modified
lipid in the direction of the oppositely charged electrode.
Moreover, streptavidin mobility could be modulated. This
method paves the way for further development of
analytical devices.