Elmar Gool

 

PhD Student AMC Medical Research BV

 

 

E-mail:

e.l.gool@amc.uva.nl

 

 

Telephone:

+31 53 489 5220

Fax:

+31 53 489 3511

 

 

Address:

Department of Biomedical Physics and Engineering (BMEP) & Department of Clinical Chemistry (LEKC)

Building: Academisch Medisch Centrum

Room: K01-226

Meibergdreef 9

P.O. Box 22700

1100 DE Amsterdam

The Netherlands

Project goals:

Sensitive detection of EV by SPRi.

Surface Plasmon Resonance Imaging

Surface Plasmon Resonance Imaging (SPRi) can detect the plasmon resonance angle at an interface. If this interface consista of borosilicate glass below, coated with a gold nanolayer and a liquid sample on top. The refractive index contrast at the interface determines this resonance angle, and thus can be used to measure the change in refractive index above the interface. With a camera, the resonance angle can be determined for many positions on a chip simultaneously. Shifts in resonance angle in the order of mili-radians can be detected (refractive index changes in the order of 10-6). First results suggest that some antigens (e.g. EpCAM, CD44) are conserved from cells to EV, while others (e.g. Her3) are not.

Tasks:

Y1 optimizing system and protocols, Y2: understand response curves, investigate sorting, Y3: Model samples, multiplex antibodies, Y4: Patient samples.

Collaboration:

IBIS Technologies, Enschede

Medical CellBioPhysics, University of Twente

Sanquin, Amsterdam

SSens, Enschede

Figure 1) SPR gold coated surface on which proteins can be printed for interaction measurements.

Figure 2) Antibody print on the interface which detects membrane antigens in the EV sample.