REDUCIBLE VINYL POLYMERS FOR DRUG AND GENE DELIVERY

BACKGROUND

The development of new non-viral drug and gene delivery vehicles has made tremendous progress over the past decade. Cationic polymers such as polyethyleneimine (PEI) and poly(2-dimethylaminoethyl methacrylate) (PDMAEMA) have proven very effective transfection agents, since they readily form stable polyplexes with for example siRNA and easily penetrate the cell wall to deliver their cargo.

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A major drawback, however, to these polymers is their persistent nature. The main polymer chain, also called backbone, of PDMAEMA consists of carbon-carbon bonds. The recent development of cyclic monomers for radical polymerization may provide a solution to this problem. These cyclic monomers may be added to an existing recipe for PDMAEMA polymers, and whichever functional group is present in the cyclic monomer, will be incorporated into the main polymer chain during the polymerization.

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AIM OF THE PROJECT

The monomer 2-dimethylaminoethyl methacrylate will be polymerized via controlled radical polymerization techniques, in the presence of a disulfide-functional cyclic monomer. Polymers of different lengths and containing different amounts of cyclic monomer will be prepared and fully characterized. Their degradation will be studied and, depending on available time and experience, their efficacy in DNA delivery will be studied.

 

APPROACH

Initially, we will prepare PDMAEMA polymers of different length, without the cyclic monomer. A disulfide-functional cyclic monomer will be prepared via a 3-step synthesis, and the products will be characterized with NMR and infra-red spectroscopy. PDMAEMA polymers with the cyclic monomer will be prepared, and these polymers will also be fully characterized. Finally, their degradation will be studied as well as their efficacy in DNA delivery.

CONTACT INFORMATION

 

DR. JOS PAULUSSE

PROF. JOHAN ENGBERSEN

 

DEPARTMENT OF BIOMEDICAL CHEMISTRY

DEPARTMENT OF BIOMEDICAL CHEMISTRY

Email:

Phone:

Room:

J.M.J.Paulusse@utwente.nl

053 - 489 3306

ZH 244

J.F.J.Engbersen@utwente.nl

053 - 489 2968

ZH 255