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Microfluidically-engineered Hydrogels for Biomedical Applications

Shoji Takeuchi*

4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, JAPAN, 1) Univ. of TOKYO 2) JST-ERATO

P:\TNW\MESA+\Users\Secretariaat\MESA+ Colloquia algemeen\Colloquia 2013\cell doll.jpgHydrogels are attractive materials because of its excellent deformability, biocompatibility, and the ability to be chemically-modified. They are thus very useful for various biomedical applications including implantable health monitoring and tissue engineering. In this presentation, I will speak about several MEMS/Microfluidic-based approaches for the rapid and reproducible assembling of hydrogel microstructures and their biomedical applications.

Fluorescent hydrogels hold great promise for in vivo continuous glucose monitoring with wireless transdermal transmission and long-lasting activity. We synthesized a highly-sensitive fluorescent monomer, and then fabricated injectable-sized fluorescent polyacrylamide hydrogel beads and fibers with high uniformity and high throughput. We find that the fluorescent beads provide sufficient intensity to transdermally monitor glucose concentrations in vivo.

Large-scale 3D tissue architectures that mimic microscopic tissue structures in vivo are very important for not only in tissue engineering but also drug development without animal experiments. We demonstrated a construction method of 3D tissue structures by using cell laden hydrogel microbeads and microfibers. We believe that this method is useful to create complex structures with multiple types of cells that functions as a living organism.

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References :

1. Hiroaki Onoe, Teru Okitsu, Akane Itou, Midori Kato-Negishi, Riho Gojo, Daisuke Kiriya, Koji Sato, Shigenori Mirua, Shintaroh Iwanaga, Kaori Kuribayashi-Shigetomi, Yukiko Matsunaga, Yuto Shimoyama, and Shoji Takeuchi: Metre-long Cell-laden Microfibres Exhibit Tissue Morphologies and Functions, Nature Materials, 2013 doi:10.1038/nmat3606

2. Yun Jung Heo , Hideaki Shibata , Teru Okitsu , Tetsuro Kawanishi, and Shoji Takeuchi: Long-term in vivo glucose monitoring using fluorescent hydrogel fibers, PNAS, vol. 108(33):13399, 2011

3. Y. Matsunaga, Y. Morimoto and S. Takeuchi, Bead-based tissue engineering: moulding cell beads into a 3D tissue architecture, Advanced Materials, 23(12): H90, 2011.

4. H. Shibata, YJ. Heo, T. Okitsu, Y. Matsunaga, T. Kawanishi, and S. Takeuchi: Injectable hydrogel microbeads for fluorescence-based continuous glucose monitoring, PNAS, 107(42):17894, 2010

5. N. Misawa, H. Mitsuno, R. Kanzaki, S. Takeuchi: A Highly Sensitive and Selective Odorant Sensor using Living Cells Expressing Insect Olfactory Receptors, PNAS, 107(35): 15340, 2010

6. W-H. Tan and Shoji Takeuchi: A Trap-and-Release Integrated Microfluidic System for Dynamic Microarray Applications, PNAS, 104(4): 1146, 2007

7. Shoji Takeuchi, Piotr Garstecki, Douglas B. Weibel, and George M. Whitesides: An Axisymmetric Flow-Focusing Microfluidic Device, Advanced Materials, 17(8): 1067, 2005.

Keywords: Microfluidics, Lab on a Chip, 3D tissue construction, Implantable Sensors


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