The MAC-Twente facility offers a wide variety of optical microscopy techniques for highly detailed imaging and analysis of biological and non-biological specimens. Live cell imaging facilities are available, both for stand-alone microscopy and integrated with automated liquid handling, enabling fully automated screening applications using living cells. The available STORM and NSOM techniques allow imaging with enhanced optical resolution. Each microscope system incorporates different imaging modalities for simultaneous or sequential visualisation of different sample characteristics. Dedicated software packages are available for comprehensive image analysis.