MESA+ University of Twente
MESA+ NanoLab

As far as light goes

Optical Microscopy

The MAC-Twente facility offers a wide variety of optical microscopy techniques for highly detailed imaging and analysis of biological and non-biological specimens. Live cell imaging facilities are available, both for stand-alone microscopy and integrated with automated liquid handling, enabling fully automated screening applications using living cells. The available STORM and NSOM techniques allow imaging with enhanced optical resolution. Each microscope system incorporates different imaging modalities for simultaneous or sequential visualisation of different sample characteristics. Dedicated software packages are available for comprehensive image analysis.


  • Scanning confocal

  • Combination with AFM

  • Combination with NSOM


·    Wide field

         - combination with: bright field and Differential Interference Contrast (DIC),

         - Combination with Atomic Force Microscopy (AFM) and Fluid Force Microscopy (FluidFM)

· Scanning confocal

                - Combination with transmitted light and DIC

                - Combination with AFM

· Total Internal Reflection Fluorescence (TIRF)

· Super-resolution – Stochastic Optical Reconstruction Microscopy (STORM)

· Automated spinning disk confocal

                - Combination with bright field and Digital Phase Imaging (DPI)

                - Integrated with automated liquid handling (ALH)

                - Server based data storage and analysis

· Near-field scanning optical microscopy (NSOM)

· Live cell imaging facilities