Chair: Mathieu Odijk (BIOS lab on chip group)
Short introduction by Mathieu Odijk (BIOS)
Yali Zhang (SFI)
Performance study of pervaporation in a microfluidic system for the removal of acetone from water
Hoon Suk Rho (MCS)
pH gradient chromatofocusing on a chip
Floris van den Brink (BIOS)
Microreactor with integrated boron doped diamond electrodes for electrochemical protein cleavage and drug screening
Jos Quist (NI)
Fluidic force microscopy for single cell analysis
Performance study of pervaporation in a microfluidic system for the removal of acetone from water - Yali Zhang (Soft Matter, Fluidics and Interfaces group)
We aim to conduct a performance study for the removal of acetone from water using a continuous micro-pervaporation system. High acetone removal efficiency of 84% is achieved for just 4 minutes residence time at room temperature. We have derived a design criterion based on the intrinsic system parameters to engineer the pervaporation system for both micro- and macro-scales.
pH gradient chromatofocusing on a chip – Hoon Suk Rho (Mesoscale Chemical Systems)
We have developed a novel microfluidic platform for pH-gradient chromatofocusing of proteins by adapting 16 parallel micro-mixers, a micro-column, and a multiplexer. The creation of 16 non-linear gradients and the generation of a solid-phase micro-column have been achieved for the realization of anion exchange chromatography on a single chip. With the device we demonstrated the separation of a protein mixture of R-phycoerythrin and FITC-BSA based on pH-gradient chromatofocusing. We believe that the fully automated and integrated microfluidic platform for high-throughput protein screening could be considered as a promising approach for fast biopharmaceutical process development.
Microreactor with integrated boron doped diamond electrodes for electrochemical protein cleavage and drug screening – Floris van den Brink (BIOS Lab on a Chip Group)
Electrochemical cells on chip have shown potential for use in both proteomics studies and drug screening, and in this presentation we highlight these areas of application. With our latest developments we aim to exploit the benefits of boron doped diamond as a novel electrode material to conduct tyrosine- and tryptophan-specific electrochemical protein cleavage. In addition, we mimic phase I drug metabolism on chip and we were able to detect short-lived and reactive metabolites, demonstrating a valuable assay for early drug discovery processes.
Fluidic force microscopy for single cell analysis – Jos Quist (NanoIonics)
A force microscope with integrated microfluidic probe was used to study the waterborne pathogen Cryptosporidium with a throughput of >50 cells/hr. Whereas conventional microscopy techniques cannot discern non-viable pathogens from viable ones, force measurements revealed a loss of elastic anisotropy after heat treatment. Currently, we are extending the platform with a micro/nanofluidic chip for subcellular sampling.