Safety instructions BSS labs ZH193-Z188

Protocol BSS Labs: Safety and Lab usage ZH187, ZH188, ZH190, ZH193.

General

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Leave coats and backpack outside the lab

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Wearing safety glasses and lab coat is obligatory when using hazardous chemicals. Wearing Lab coat is obligatory in ZH188. Take off your lab coat upon leaving the lab. No eating or drinking in any of the labs.

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No smoking in the lab (or anywhere in the building).

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When using chemicals with the H-phrase 300, 310, 330 filling in a Red Safety card is obligatory. This Red Safety card has to be placed outside the lab in the proper holder together with all the MSDS sheats and a copy of the yellow card.

Please mind to put your personal telephone number on the cards where you can be reached during and outside working hours!

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There are lab tables present. They are for general use; therefore they need to be cleaned after every usage. No working with organic solvents on the lab tables: fume hood only.

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Take off your gloves before you pick up the telephone, the door handle or your pen. Immediately remove the gloves in case of spilling chemicals with the following signs :

and clean your hands with water and soap.

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It is obligatory be instructed before you use equipment for the first time. The equipment supervisor will explain the rules you have to comply to.

Failing to do so may result in banishment from that equipment.

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It is only allowed to remove items from the lab when the lab manager has been consulted.

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Regularly wash your hands and always wash your hands before leaving the lab. No running in the labs or hallways, when crossing other people, keep in mind that those who carry chemicals or equipment have the advantage.

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Don’t block the escape routes, entrances, fire extinguishers, eye/emergency shower etc.

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Try to keep the lab doors closed to maintain the needed underpressure in the labs.

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At the end of the day check if everything is as clean as it was, when you entered the room! Switch off the lights & close the doors.

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At the end of the contract, all lab users are obliged to leave their working space clean behind. All chemicals and samples must be removed or officially passed on to another lab user. As soon as you don’t need them anymore, but not later then the ending of the contract, inform the lab manager which chemicals you removed and which you passed on and to whom.

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Gas cylinders may only be connected by a qualified person.

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Additional information(dutch) regarding safety and environment can be found at: http://www.tnw.utwente.nl/intra/diensten/amh/amh_ct/

Cell culture rules

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Leave coats and backpack outside the lab

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Wearing Lab coat is obligatory in ZH188.

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Take off your lab coat upon leaving the lab.

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No eating or drinking in any of the labs.

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Wearing safety glasses and lab coat is obligatory when using hazardous chemicals.

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Wash your hands before start and at the end.

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Wear gloves when handling cells and spray them regularly with 70% ethanol

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Spray and wipe the workspace of the culture hood with 70% ethanol before and after use

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Spray and wipe equipment & solutions before putting them in the hood

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Discard disposable plastics and toxic waste in the blue-yellow bin

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Discard non-toxic liquids in the sink

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Discard gloves and tissues in the normal waste

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Sharps can be discarded in the yellow sharps box

Chemicals

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Be aware of the possible dangers and necessary precautions (fume hood, safety glasses, gas mask, gloves or additional measures). Also keep your direct area in mind when setting up new experiments. Read the quick guide “Hazard & precautionary statements” attached to this document.

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Use a clean spatula every time you weigh something from the stock flask. Never put anything you spilled back in the bottle. Clean balances after each weighing.

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Never let a flask standing open and always clean up spilled materials immediately!

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Return chemicals in the grey cupboards/freezer/fridge immediately after you finished using them.

Glassware

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All glasswork is for general use. Always clean your glassware immediately after you finished with it. This means washing with warm Biotex, rinsing with warm water, rinsing with 70% ethanol placing it into the cupboard or appropriate place upside down.

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Broken glasswork can be thrown away in the orange containers in the hallway. Empty sample bottles and disposable test tubes need to be cleaned before throwing them away. This prevents nasty smells of chemicals or health risks in the direct environment à to be arranged

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Always put your name, date, phone nr and contents on bottles containing samples before storing them.

Stock and saving

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Chemicals and equipment can be ordered by the lab manager.

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Chemicals, tissues, plastic bottles etc. can be found in ZH190. Whenever taking supplies fill in the list in ZH190 and report to the lab manager as well

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Do not store chemicals or samples with unreadable marking. Make sure that the bottles are sealed correctly.

Waste

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“Clean” waste, including paper and packaging material goes in the grey containers. This is also allowed for clean polymers.

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Sharp waste, such as needles and razor blades in the special yellow boxes.

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Chemical waste is put in jerry cans, organized with respect to the category of the waste. The storage is in the fume hood in lab ZH278. Check before the experiment which category waste you will produce.

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Solid chemical and biological waste can be discarded in the blue bins as long as they are not reactive, smelly or dangerous in any way. In case of fine particles(dust), prepack the waste in a plastic sealed bag.

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Glass needs to be thoroughly cleaned/rinsed before throwing it in the orange container.

Fume hood

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Always make sure that the ventilation is working. It’s forbidden to use a fume hood with a disrupted ventilation

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Set up your experiment as far back in the fume hood as possible. Be sure to tighten the parts properly

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Keep the fume hood clean and not too crowded. Place water and oil baths on a labjack to enhance a good circulation of air.

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Keep the window down as much as possible and never put your head in the fume hood. Do not make sudden movements in front of the fume hood

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Be sure to tighten connections to water outlets properly to prevent sudden disconnections.

Emergency

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Always be aware of the location of the emergency showers, eye showers, fire extinguishers (and their application), emergency exits and meeting place after an incident.

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When chemicals have contacted the skin always rinse with a lot of water. In case of symptoms always check a doctor (telephone number 2222).

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In case of an emergency dial the alarm number 2222.

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In case of an evacuation make sure that the equipment you leave behind is safe.

Close the windows and doors, follow the instructions and go to the meeting place. In case of fire don’t use the elevators.

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It is obligatory to report dangerous situations or (almost) accidents to the Lab manager and AMC

Outside office hours

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One can apply for activation of the entrance authorization outside office hours in order to be able to enter or leave the building. Check with Marcel Weusthof room ZH230 for more information.

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It is not allowed to work alone in the lab.

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It is not allowed to perform dangerous experiments outside working hours.

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If you need to perform certain tasks in the lab outside working hours, be sure that you are at least with two qualified persons. It’s not allowed to take friends and/or family with you.

The lab user will work in the BSS labs according to this protocol.

Name, signature & date Name, signature &date

lab user. AMC/Lab chief.

February 6, 2014