ML-1 / ML-2 labs
ML-1 /ML-2 classified labs
General
Within our department we have 4 Microbiological Laboratories (ML):
- Cr4.430 is classified as a biochemical ML-1 laboratory.
- Cr4.426 is classified as a bacteriological ML-2 laboratory.
- Cr4.420 is classified as a cell culture ML-2 laboratory.
- Cr4.319 is classified as a fungus ML-2 laboratory.
Before starting a microbiological project, a risk analysis needs to be established (RIE) based on the information about the micro-organism (bio-safety level), amounts & volumes used, aerosol production, materials used etc) and it needs to be covered by the permit.
Contact Regine van der Hee for more information.
Project members
All doors to the GMO labs remain closed during all experiments. Access to these labs is restricted to those who are aware of the risks of entering the room. GMO-activities are restricted to project members or employees/students working under the supervision of project members. Dedicated lab coats are obligatory (and not allowed to be worn outside this lab!). Hands must be washed upon entering and before leaving this lab. Access can be granted after an introduction by Regine van der Hee. Everybody needs to have a GMO registration by filling in the GMO approval form for consent of the biological safety officer. Registered persons have unlimited access.
Waste
Liquid waste (ML-1/ ML-2) is collected in a marked conical flask with sufficient amount of fresh bleach (~10%), as indicated on the flask. After at least 30 min of incubation with bleach, the flask can be emptied in the sink.
Solid waste with potentially contaminated materials is collected in the blue bins with single closure yellow lids and labeled accordingly (Ziekenhuis- or GGO-afval). Every 2-3 weeks or when full, the bins are collected by technicians for destruction by a certified company.
Contaminated material
Re-used material needs to be decontaminated with 70% ethanol or autoclaved. After decontamination the material can be washed according to the normal procedure. Bring it to the dishwasher yourself.
Cleaning
A dedicated lab coat has to be worn during all activities. Normal cleaning activities (floors, doors & sink) are performed by trained and dedicated cleaning personnel (registered). Cleaning work benches is not included in the activities of the cleaning personnel. Extra cleaning activities are performed by the lab members themselves following a corvee-list available inside all ML-1 / ML-2 labs.
Duty list project members
Cleaning tasks:
- clean bench with 70% ethanol daily
- clean waterbath with alconox every 6 months
- clean shaker with 70% ethanol monthly
- clean centrifuges with 70% ethanol monthly
- disposal of contaminated solid waste bin biweekly
If you have questions or if you are not able to fulfill your tasks please contact Regine van der Hee or Pascal Jonkheijm.