Telephone: +31 53 489 5362
Fax: +31 53 489 3511
Address: Faculty of Science and Technology
Medical Cell BioPhysics (MCBP)
P.O. Box 217
7500 AE Enschede
Genetic and phenotypic characterization of Circulating Tumor Cells (CTC) offers the opportunity for a “real time liquid biopsy”. Heterogeneity and rarity of CTC command the need for individual cell characterization. Following an enrichment procedure of CTC from blood, the identification, isolation and manipulation of single cells for further analysis without cell loss remains challenging. Microfluidic device is a powerful tool to improve genetic and phenotypic analysis of single cells by making use of the smaller reagent volume, automation and parallel reaction.
Develop and optimize microfluidic devices for single cancer cell characterization such as single cell isolation, lysis, DNA amplification, and drug response monitoring.
Figure 1.(Left) Real photo of a microfluidic device for single cell whole genome amplification (Right) Schematic view. Single cell was isolated, lysed, and their DNA were amplified on a chip. Amplified DNA were validated using qPCR targeting template-specific genes such as ERBB2. PLoS ONE 9(9): e107958. doi:10.1371/journal.pone.0107958
Figure 2. Schematic view of a microfluidic device with open-well structure for single cell whole genome amplification following filtration
Figure 3. Setup for operation of pneumatic microfluidic device