Research

Thrombosis in Blood Vessels-on-Chips

Thrombosis

Thrombosis is the pathological formation of blood clots inside blood vessels, even in the absence of vascular injury or bleeding. Thrombosis is the result of a combination of blood-borne factors (e.g. genetic defects in platelets or coagulation factors), vessel wall dysfunction (e.g. chronic inflammation) and fluid dynamical effects (e.g. stasis or flow reversal in stenosed valves). Thrombosis in arteries is often associated with atherosclerosis and is a major cause of myocardial infarctions and stroke.

Models of Thrombosis

Thrombosis is currently studied mostly in mouse models and by using flow chambers in vitro. Both models have their limitations, mostly because they fail to mimic key aspects of human physiology and disease.

Organ-on-chip technology is changing the way we study thrombosis in the lab. We and others have previously performed proof-of-principle experiments that demonstrate that microfluidic chips can be used to unify the three major aspects of thrombosis – blood, vessel wall, fluid dynamics – in one controlled model, and that these models can be used to study the dynamics of a forming thrombus by live-cell microscopy.

Project Goal

Q:\General\AST Website\Research\Thrombosis-on-Chip\thrombosis-on-chip.pngIn this project, we develop organ-on-chip models for thrombosis that include key aspects of thrombosis in specific patients or subsets of patients. We will develop a microfluidic chip that (1) is a direct representation of the blood vessel geometry of a specific patient, based on their angiography imaging data, (2) is covered with patient-specific endothelium that has been derived from patient stem cells, and (3) is perfused with patient blood samples. We will apply these patient-specific organ-on-chip models of thrombosis to understand and predict the risk of thrombosis for specific subsets of patients, as well as to evaluate drug and treatment efficacy for these patients.

This project is a collaboration with the BIOS/Lab on a Chip group of the University of Twente, and is part of the ERC Advanced VESCEL program, headed by prof. Albert van den Berg.

For more information, please contact:

Hugo Albers
PhD candidate

Info

+31-(0)53-489 5134
 H.J.Albers@utwente.nl
Andries van der Meer
Assistant Professor, Director MIRA