A major determinant of heterogeneity in tumours is the constantly changing mutational landscape of individual cancer cells due to the accumulation of new mutations. One of the challenges in the field is to determine the rate and extent of genetic alterations per cell cycle of individual cancer cells.
With the joint effort of the UMCU (biology) and UT (engineering), we will develop microfluidic tools and protocols to integrate prospective single-cell genetic analysis with live-cell microscopy to determine the rate and extent of genetic alterations per cell cycle of individual cancer cells.
- Yannik Bollen (Medical Cell BioPhysics – UT)
- Dr. Hugo Snippert (Molecular Cancer Research – UMCU)
- Prof. dr. Hans Bos (Molecular Cancer Research – UMCU)
- Prof. Alain de Bruijn (Pathobiology – UMCU)
- Prof. Onno Kranenburg (UCC – UMCU)
- Prof. Leon Terstappen (Medical Cell BioPhysics – UT)
- How to create state-of-the-art genetic model systems: strategies for optimal CRISPR-mediated genome editing. Bollen Y, Post J, Koo BK, Snippert HJG. Nucleic Acids Res. 2018 Jul 27;46(13):6435-6454. DOI: 10.1093/nar/gky571 https://www.ncbi.nlm.nih.gov/pubmed/29955892