Lennart de Vreede
In this project a Lab on a Chip system is developed for highly sensitive detection of tuberculosis in human sputum. The system makes use of a newly developed highly sensitive optical detection method (F. Ungureanu et al. Sensors and Actuators B 150 (2010) 529-536). The work involves all aspects of the sample preparation and microfluidic chip needed for this detection method: building a setup/device for sample pretreatment, designing and manufacturing a sample flow-through reaction chamber and manufacturing a gold nanoparticle array in the reaction chamber to enable optical detection.
In a first stage of this project, the development of a gold nanobead array has led to the accidental discovery of a new method to manufacture nanopores [de Vreede et al., Nanopore Fabrication by Heating Au Particles on Ceramic Substrates, Nano Lett. 2015]. The method is both simple and versatile, and pores with lengths of up to 800 nm and diameters downto 25 nm have been produced simply by heating a SiO2 substrate with deposited gold patches in an oven.
In the present stage of the project, the flow chamber as well as sample preparation steps are being developed.
The project is being performed in cooperation with the Royal Tropics Institute (KIT, Dr. Richard Anthohy), the Nanobiophysics group of the University of Twente (Dr. Ron Gill) and Blue4Green (Erik Staijen).