Amperometric detection of hydrogen peroxide in exhaled breath condensate

The goal of this project is the development of an amperometric sensor for detection of H₂O₂ in exhaled breath condensate. H₂O₂ has been reported at elevated levels in the exhaled breath condensate of individuals affected by diseases such as chronic obstructive pulmonary disease. Typically H₂O₂ measurements are obtained off-line after collection of the exhaled breath in condensation units, which is time and labor intensive. Thus, reliable point-of-care detection has the potential to provide improvement in monitoring and treatment of affected patients. Different measurement techniques for H₂O₂ have been reported, including spectrophotometry, chemiluminescense, or electrochemical sensors. Amongst these approaches electrochemical detection is particularly attractive due to the possibility of miniaturization, cost effectiveness, and potential for mass production.

This project is structured in two stages. The first stage is aimed at development of a transducer platform, and characterization in an aqueous environment. Within this stage a chip-based three electrode structure is realized by means of microfabrication. Subsequently detection of H₂O₂through the usage of electroanalytical techniques is performed. It is found that variation of conditions such as the composition of the supporting electrolyte largely influences the obtained response. Thus care must be taken to optimize the device thoroughly. The second stage is focused on transfer of this transducer to a device capable of detecting H₂O₂in a gaseous phase, which is feasible incorporating special measures.

	Home > ... > Electrochemical systems > Amperometric detection of hydrogen peroxide in exhaled breath condensate
Home News & Highlights People Vacancies Research Education & Assignments Publications Intranet Internal Links External links Movies Sitemap Search	Emerging research topics \| Cells on chips \| Electrochemical systems \| Micro- and nanofluidics \| Nanosensing and Technology Amperometric detection of hydrogen peroxide in exhaled breath condensate The goal of this project is the development of an amperometric sensor for detection of H₂O₂ in exhaled breath condensate. H₂O₂ has been reported at elevated levels in the exhaled breath condensate of individuals affected by diseases such as chronic obstructive pulmonary disease. Typically H₂O₂ measurements are obtained off-line after collection of the exhaled breath in condensation units, which is time and labor intensive. Thus, reliable point-of-care detection has the potential to provide improvement in monitoring and treatment of affected patients. Different measurement techniques for H₂O₂ have been reported, including spectrophotometry, chemiluminescense, or electrochemical sensors. Amongst these approaches electrochemical detection is particularly attractive due to the possibility of miniaturization, cost effectiveness, and potential for mass production. This project is structured in two stages. The first stage is aimed at development of a transducer platform, and characterization in an aqueous environment. Within this stage a chip-based three electrode structure is realized by means of microfabrication. Subsequently detection of H₂O₂through the usage of electroanalytical techniques is performed. It is found that variation of conditions such as the composition of the supporting electrolyte largely influences the obtained response. Thus care must be taken to optimize the device thoroughly. The second stage is focused on transfer of this transducer to a device capable of detecting H₂O₂in a gaseous phase, which is feasible incorporating special measures.