Surface plasmon resonance imaging

Label-free biomolecular measurement techniques, such as surface plasmon resonance imaging (iSPR), are emerging as important complementary methods to conventional bioassays, which typically use fluorophore labels, because real-time binding kinetics information can be determined. Recent advances in iSPR are becoming increasingly important for label-free microarray-based assay applications where multiple, up to about 500, biomolecular interactions can be measured simultaneously. However, conventional iSPR microarray assay approaches rely on protein printing techniques for immobilization of the ligand to the gold imaging surface. The combination of microfluidics with iSPR allows the in-situ immobilization of ligands and analyte transport for the biomolecular interaction assay.

An integrated microfluidics and iSPR platform, where ligand immobilization and subsequent sample solutions are precisely positioned with an electroosmotically driven address-flow guiding technique has been demonstrated in our lab. This approach does not require complicated microfluidic channel arrangements or pressure driven transport, which requires external pumps and valves. Address-flow sample guiding is a valve-less electroosmotically driven technique used for controlling the sample stream in a microfluidic chip. The microfluidic chip arrangement (see figure below) uses the center inlet for sample introduction and upper and lower inlets for guiding. Electrical voltages V_u and V_l control the y-direction position of the sample. Voltage V_c is used to transport the sample in the x-direction. The biomolecular interactions between the sample and ligand are measured at each gold island using surface plasmon resonance.

(a) Microfabricated chip (b) iSPR gold imaging array with location (row, column) (c) iSPR chip interface module

Contact information

Dr. Edwin Carlen

BIOS Lab on a Chip Group

MESA+ Institute for Nanotechnology

University of Twente

E-mail: e.t.carlen@utwente.nl

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Home News & Highlights People Vacancies Research Education & Assignments Publications Intranet Internal Links External links Movies Sitemap Search	Emerging research topics \| Cells on chips \| Electrochemical systems \| Micro- and nanofluidics \| Nanosensing and Technology Surface plasmon resonance imaging Label-free biomolecular measurement techniques, such as surface plasmon resonance imaging (iSPR), are emerging as important complementary methods to conventional bioassays, which typically use fluorophore labels, because real-time binding kinetics information can be determined. Recent advances in iSPR are becoming increasingly important for label-free microarray-based assay applications where multiple, up to about 500, biomolecular interactions can be measured simultaneously. However, conventional iSPR microarray assay approaches rely on protein printing techniques for immobilization of the ligand to the gold imaging surface. The combination of microfluidics with iSPR allows the in-situ immobilization of ligands and analyte transport for the biomolecular interaction assay. An integrated microfluidics and iSPR platform, where ligand immobilization and subsequent sample solutions are precisely positioned with an electroosmotically driven address-flow guiding technique has been demonstrated in our lab. This approach does not require complicated microfluidic channel arrangements or pressure driven transport, which requires external pumps and valves. Address-flow sample guiding is a valve-less electroosmotically driven technique used for controlling the sample stream in a microfluidic chip. The microfluidic chip arrangement (see figure below) uses the center inlet for sample introduction and upper and lower inlets for guiding. Electrical voltages V_u and V_l control the y-direction position of the sample. Voltage V_c is used to transport the sample in the x-direction. The biomolecular interactions between the sample and ligand are measured at each gold island using surface plasmon resonance. (a) Microfabricated chip (b) iSPR gold imaging array with location (row, column) (c) iSPR chip interface module Contact information Dr. Edwin Carlen BIOS Lab on a Chip Group MESA+ Institute for Nanotechnology University of Twente E-mail: e.t.carlen@utwente.nl