Highlight: Electrokinetic Sample Focusing and Surface Plasmon Resonance Imaging System for Measuring Biomolecular Interactions

Highlight: Electrokinetic label-free screening chip: a marriage of multiplexing and high throughput analysis using surface plasmon resonance imaging

We present an electrokinetic label-free biomolecular screening chip (Glass/PDMS) to screen up to 10 samples simultaneously using surface plasmon resonance imaging (iSPR). This approach reduces the duration of an experiment when compared to conventional experimental methods. This new device offers a high degree of parallelization not only for analyte samples, but also for multiplex analyte interactions where up to 90 ligands are immobilized on the sensing surface. The proof of concept has been demonstrated with well-known biomolecular interactant pairs. The new chip can be used for high throughput screening applications and kinetics parameter extraction, simultaneously, of interactant–protein complex formation.

Fig. 1 Illustration of the biochip (top layer—PDMS and bottom layer—glass with gold islands) with chip holder (integrated with platinum electrodes as well as reservoir holes for extra sample volume). The UV curable glue (NOA-81) bonded chip is also shown here. Top left side image represents the electrical connections (for example: channel 1 and channel 7 are connected to the same voltage source). A–A’ (grey) line represents the splitting of two groups of channels structures and is also shown in chip images.

A detailed description can be found in the communication paper published in Lab-on-a-Chip Journal “Advanced Article” section on 9^th March 2010: http://www.rsc.org/publishing/journals/LC/article.asp?doi=C000705F

For more information please contact Ganeshram Krishnamoorthy: g.krishnamoorthy@utwente.nl