Highlight March 2008: Nano2Life Poster Award for Georgette Salieb-Beugelaar

At the annual meeting of Nano2Life in Champery (Switzerland) the poster presenting the recent DNA work has been awarded with the third price. Part of this work was funded by Nano2Life.

Field Dependent Mobility of DNA in 20 nm High Nanoslits

The transport behavior of λ-DNA (48 kbp) in fused silica nanoslits is investigated upon application of electrical fields of different strength. The slit dimensions are 20 nm in height, 3 µm in width and 500 µm in length. With fields of 30 kV/m or below, the molecules move fluently through the slits while at higher electrical fields, the DNA molecules move intermittently resulting in a strongly reduced mobility. Preferential pathways and trapping sites of the λ-DNA molecules were found throughout the nanoslits which indicates that the classical viscous friction models to explain the DNA movement in nanoslits needs to be modified.

Right: the surface roughness of the nanoslits as measured by AFM and using tips with a radius of 2 nm. Although the rms value is between 0.8 nm – 1.1 nm, holes occur with a depth of down to 8 nm.

Left: an example of a typical analysis is presented here in three frames. The tracked DNA is inside the black circle. The color coding runs from green (high fluorescent emission) to red (low fluorescent emission). Out of these data, the mobility of the DNA was calculated.

A paper with this work was submitted at Nano Letters.

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Home News & Highlights People Vacancies Research Education & Assignments Publications Intranet Internal Links External links Movies Sitemap Search	weblink \| News archive \| Archive Highlights \| Promotie film Ana Valero Highlight March 2008: Nano2Life Poster Award for Georgette Salieb-Beugelaar At the annual meeting of Nano2Life in Champery (Switzerland) the poster presenting the recent DNA work has been awarded with the third price. Part of this work was funded by Nano2Life. Field Dependent Mobility of DNA in 20 nm High Nanoslits The transport behavior of λ-DNA (48 kbp) in fused silica nanoslits is investigated upon application of electrical fields of different strength. The slit dimensions are 20 nm in height, 3 µm in width and 500 µm in length. With fields of 30 kV/m or below, the molecules move fluently through the slits while at higher electrical fields, the DNA molecules move intermittently resulting in a strongly reduced mobility. Preferential pathways and trapping sites of the λ-DNA molecules were found throughout the nanoslits which indicates that the classical viscous friction models to explain the DNA movement in nanoslits needs to be modified. Right: the surface roughness of the nanoslits as measured by AFM and using tips with a radius of 2 nm. Although the rms value is between 0.8 nm – 1.1 nm, holes occur with a depth of down to 8 nm. Left: an example of a typical analysis is presented here in three frames. The tracked DNA is inside the black circle. The color coding runs from green (high fluorescent emission) to red (low fluorescent emission). Out of these data, the mobility of the DNA was calculated. A paper with this work was submitted at Nano Letters. See also: http://www.nano2life.org/news.php?nid=35 Georgette B. Salieb-Beugelaar Twente University EWI/BIOS Hogekamp 45 Postbus 217 7500 AE Enschede The Netherlands. Phone +31 53 489 2755 Mobile +31 6 147 12013 Fax +31 53 489 3595 g.b.salieb-beugelaar@ewi.utwente.nl http://bios.ewi.utwente.nl/people/phdstudents/Salieb.doc/index.html