Highlight: Electrokinetic Lab-on-a-BioChip for Multi-Ligand/Multi-Analyte Biosensing

Electrokinetic Lab-on-a-BioChip for Multi-Ligand/Multi-Analyte Biosensing

We present a simple electrokinetic lab-on-a-biochip (EKLB) with four microchannels integrated with a surface plasmon resonance imaging (iSPR) label-free biosensor that is operated using a single electrical voltage for the simultaneous transport of reagents in all microchannels without conventional fluidic plumbing. We demonstrate the utility of the simple approach with various biosensing experiments, including single injection kinetics (multiple varied ligand densities and single analyte concentration), one shot kinetics (single ligand densities and multiple varied analyte concentrations), and multi-ligand/multi-analyte detection. In all cases, the binding kinetics and affinity were extracted using a conventional 1:1 interaction model. Since the reagent transport is done with a single electrical voltage source, scaling up to hundreds to thousands of simultaneous experiments is straightforward.

Fig. 1 (a) 6×4 microarray of gold iSPR sensing islands (b) 15×15 mm² glass-PDMS microfluidic chip showing channels C₁ - C₄. S₁ – S₄ are sample fluidic inlets. (c) iSPR interface module with chip fixture with integrated platinum electrodes. (d) Electrical circuit representation of the EKLB chip. R₁ - R₄ represents electrical resistances in the respective channels. I₁ - I₄ represents electrical current in the respective channels. O represents the common fluidic outlet and electrical ground.

A detailed description can be found in the research paper published in Analytical Chemistry Journal “ASAP” section on 19^th April 2010: http://dx.doi.org/10.1021/ac1003163

For more information please contact Ganeshram Krishnamoorthy: g.krishnamoorthy@utwente.nl